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PMID: 6449653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

On the nature of sbcA mutations in E. coli K 12.

Molecular & general genetics : MGG ·Vol. 179 ·No. 3 ·1980-00-00 ·Pages 555-63

Kaiser K, Murray NE

Abstract

We have recently shown (Kaiser and Murray 1979) that many E. coli K 12 strains carry a defective prophage (Rac) located a few minutes clockwise of the trp operon on the genetic map. The Rac genome contains recE, the determinant for the ATP-independent exonuclease, ExoVIII. E. coli K 12 strains which carry sbcA mutations express recE constitutively. This paper describes an investigation of several such strains. We show that the SbcA phenotype may arise from more than one type of mutational change. The most readily explained SbcA phenotype is that of sbcA8 strains in which a large section of the Rac genome (including one hybrid attachment site and probably the prophage repressor gene) is deleted. Three sbcA- strains carry multiple (and probably tandemly repeated) copies of the Rac genome while two others carry a single Rac prophage that is indistinguishable in its hybridisation behaviour from that carried by sbcA+ strains.

MeSH Terms
Bacteriophage lambda/genetics Chromosome Deletion Chromosomes, Bacterial DNA, Bacterial/genetics DNA, Viral/genetics Escherichia coli/genetics Mutation Phenotype Recombination, Genetic Suppression, Genetic
Chemicals
DNA, Bacterial DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaiser K
Murray N E
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271745
Published
1980-00-00
Pages
555-63
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271745
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