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PMID: 4610579 Published · ppublish English Journal Article

Isolation of exonuclease VIII: the enzyme associated with sbcA indirect suppressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3593-7

Kushner SR, Nagaishi H, Clark AJ

Abstract

recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed by the presence of sbcA(-) mutations. sbcA(-) strains contain an increased level of an ATP-independent nuclease. Genetic and enzymatic tests indicate that this activity is not exonuclease III, exonuclease V (recB-recC nuclease), DNA polymerase I, or lambda exonuclease. This new enzyme (exonuclease VIII) has been purified 750-fold and shows a striking preference for double-stranded DNA over heat-denatured DNA. It does not act endonucleolytically on closed circular, single-stranded DNA as exonuclease V does. It also lacks a 3'-phosphatase function. Analysis on sodium dodecyl sulfate-polyacrylamide gels indicates that exonuclease VIII is not present in unsuppressed (sbcA(+)) strains. It is thought that sbcA determines some type of control function; the structural gene for exonuclease VIII is denoted by recE.

MeSH Terms
Chromatography, DEAE-Cellulose DNA, Single-Stranded/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Exonucleases/immunology,isolation & purification Genes Hydrolysis Mutation Nucleic Acid Denaturation Recombination, Genetic Suppression, Genetic Ultracentrifugation
Chemicals
DNA, Single-Stranded Exonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kushner S R
Nagaishi H
Clark A J
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3593
Published
1974-09-00
Pages
3593-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433821
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3593 PMC Full Text View journal details
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