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PMID: 160489 Published · ppublish English Journal Article

Loss of rac locus DNA in merozygotes of Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 175 ·No. 3 ·1979-10-01 ·Pages 245-50

Evans R, Seeley NR, Kuempel PL

Abstract

DNA-DNA hybridization was used to demonstrate that the substituted DNA in the bacteriophage lambda recE (formerly called lambda reverse) is homologous to DNA at the rac locus in Escherichia coli. Strains that are rac- do not contain appreciable amounts of this DNA, and it is lost from a rac+ episome (F' 123) after transmission to a rac- recipient. This is consistent with the proposal that the rac locus contains a cryptic prophage (Low, 1973).

MeSH Terms
Bacteriophage lambda/genetics Conjugation, Genetic DNA, Bacterial/genetics DNA, Viral/genetics Escherichia coli/genetics Genes Genotype Nucleic Acid Hybridization Phenotype Recombination, Genetic
Chemicals
DNA, Bacterial DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Evans R
Seeley N R
Kuempel P L
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00397223
Published
1979-10-01
Pages
245-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00397223
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