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PMID: 350855 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2.

Journal of bacteriology ·Vol. 134 ·No. 3 ·1978-06-00 ·Pages 902-12

Kuempel PL, Duerr SA, Maglothin PD

Abstract

Escherichia coli CRT4624-P2sig5 is a dnaA mutant in which integration of the prophage P2sig5 has occurred at the attP2II site (min 85). This strain was integratively suppressed, and when cells were shifted to 42 degrees C replication was initiated at a site in or near the P2 prophage. Initially, this replication occurred primarily in the direction that corresponds to the clockwise direction on the genetic map. Replication also occurred in the counterclockwise direction, but the initiation of replication in this direction occurred approximately 40 min later than the initiation of replication in the other direction. Because of this delay, the replication forks that traveled in the clockwise direction were the first to arrive in the region of the replication terminus. These replication forks ceased replication near the aroD locus (min 37), and it is proposed that the replication terminus is between the aroD and rac loci (min 31). A model is proposed for the cycle of chromosome replication in this strain at 42 degrees C.

MeSH Terms
Chromosomes, Bacterial Coliphages/genetics DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics,metabolism Lysogeny Mutation Suppression, Genetic Temperature
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuempel P L
Duerr S A
Maglothin P D
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.3.902-912.1978
Published
1978-06-00
Pages
902-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222337
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.3.902-912.1978 PMC Full Text View journal details
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