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PMID: 333449 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Terminus region of the chromosome in Escherichia coli inhibits replication forks.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 9 ·1977-09-00 ·Pages 3927-31

Kuempel PL, Duerr SA, Seeley NR

Abstract

Induction of prophage P2sig5 at 42 degrees caused replication of the bacterial chromosome in a dnaA mutant of Escherichia coli. The P2sig5 is integrated in this strain near the metG locus, which is at min 47 on the genetic map. The regions of the chromosome replicated after prophage induction have been determined by means of DNA-DNA hybridization with various DNAs obtained from Proteus mirabilis/E. coli F' merogenotes and from lambda specialized transducing phage. The replication was initiated at the prophage site and was bidirectional. Most of the replication occurred in a counterclockwise direction on the genetic map, and the replication quickly proceeded to the aroD locus (min 37). The replication forks were retarded between aroD and rac (min 31) loci, although the rac locus was finally replicated. A more severe inhibition of replication occurred between the rac and trp (min 27) loci. It is proposed that the replication terminus is near the rac locus and that the terminus inhibits replication forks.

MeSH Terms
Chromosomes, Bacterial/metabolism Coliphages/metabolism DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/metabolism Nucleic Acid Hybridization Transduction, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuempel P L
Duerr S A
Seeley N R
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.9.3927
Published
1977-09-00
Pages
3927-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431788
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.9.3927 PMC Full Text View journal details
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