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PMID: 6906662 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem.

Nucleic acids research ·Vol. 8 ·No. 22 ·1980-11-25 ·Pages 5213-22

de Bruijn MH, Schreier PH, Eperon IC, Barrell BG, Chen EY, Armstrong PW, Wong JF, Roe BA

Abstract

A unique transfer RNA has been identified in human and bovine mitochondria that lacks the "dihydrouridine" loop and stem structure. This tRNA is mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the RNA shows that it is post-transcriptionally modified by the addition of CCA at the 3' terminus and that at least one base is modified. As predicted by its anticodon (GCU, corresponding to the serine codons AGU/C) this tRNA can be aminoacylated with serine when purified mitochondria are incubated in a medium containing 3H-serine.

MeSH Terms
Animals Anticodon Base Sequence Cattle Cloning, Molecular DNA, Recombinant/metabolism Humans Mitochondria, Liver/metabolism Nucleic Acid Conformation Plasmids RNA, Transfer, Amino Acyl/analysis,biosynthesis Species Specificity Uridine/analogs & derivatives,analysis
Chemicals
Anticodon DNA, Recombinant RNA, Transfer, Amino Acyl 5,6-dihydrouridine Uridine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
de Bruijn M H
Schreier P H
Eperon I C
Barrell B G
Chen E Y
Armstrong P W
Wong J F
Roe B A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.22.5213
Published
1980-11-25
Pages
5213-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324296
Subset
IM
Grants
NIGMS NIH HHS · GM-00124 · United States
NIGMS NIH HHS · GM-21405 · United States
NIGMS NIH HHS · GM-25962 · United States
Databases
GENBANK
K00364, K00365
External Links
PubMed source DOI: 10.1093/nar/8.22.5213 PMC Full Text View journal details
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