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PMID: 6889133 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Chromatin assembled in the presence of cytosine arabinoside has a short nucleosome repeat.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5451-66

Leffak IM

Abstract

Incubation of MSB cells with cytosine arabinoside (1-beta-D-arabinofuranosylcytosine, ara-C) inhibits 3H-thymidine incorporation into nascent DNA while nucleosome core histone synthesis proceeds in molar stoichiometry at about 20% of control rates. The excess nascent histone is incorporated into chromatin and nucleosome cores are assembled normally on the small amount of DNA which is synthesized at submaximal levels of ara-C. This DNA becomes packaged into a shortened nucleosome repeat, however. These results indicate that the nucleosome core is a strongly conserved unit of chromatin replication and suggest that the stoichiometry of nascent histone to DNA may be one factor influencing the establishment of the nucleosome repeat length. It cannot be the only factor, however, since the closely packed nucleosomes made in the presence of ara-C begin to return to their normal spacing within six hours after reversal.

MeSH Terms
Cell Line Chromatin/drug effects,metabolism Cytarabine/pharmacology DNA Replication/drug effects Histones/genetics,isolation & purification Kinetics Molecular Weight Nucleosomes/drug effects,metabolism Repetitive Sequences, Nucleic Acid
Chemicals
Chromatin Histones Nucleosomes Cytarabine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Leffak I M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5451
Published
1983-08-25
Pages
5451-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326290
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.16.5451 PMC Full Text View journal details
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