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PMID: 6856473 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stability of the conservative mode of nucleosome assembly.

Nucleic acids research ·Vol. 11 ·No. 9 ·1983-05-11 ·Pages 2717-32

Leffak IM

Abstract

The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core histones. Moreover, when DNA synthesis is inhibited by ara-C nucleosome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.

MeSH Terms
Animals Cycloheximide/pharmacology DNA/metabolism DNA Replication Histones/biosynthesis,metabolism Nucleosomes/ultrastructure
Chemicals
Histones Nucleosomes DNA Cycloheximide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Leffak I M
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.9.2717
Published
1983-05-11
Pages
2717-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325919
Subset
IM
Grants
NIGMS NIH HHS · GM26803 · United States
External Links
PubMed source DOI: 10.1093/nar/11.9.2717 PMC Full Text View journal details
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