Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes.

Nucleic acids research ·Vol. 8 ·No. 12 ·1980-06-25 ·Pages 2787-805

Albanese I, Weintraub H

Abstract

Monomer nucleosomes from chick erythrocytes can be fractionated according to their electrophoretic mobility in (comparatively) high salt acrylamide gels. We show that the fractionation is based predominantly on differences in charge. The monomer heterogeneity persists even when the nucleosomes are trimmed down to 145 bp with Exo III or when H1 and H5 are removed. The slowest migrating monomers are associated with HMG 14 and 17; however, we do not believe that these proteins are entirely responsible for the altered mobility since the nucleosome heterogeneity persists even after removal of HMG 14 and 17. The DNA associated with the HMG 14 and 17 containing nucleosomes is shown to be enriched in actively transcribed globin sequences.

MeSH Terms

Animals Cell Fractionation Chickens Chromosomal Proteins, Non-Histone/blood Electrophoresis, Polyacrylamide Gel/methods Erythrocytes/analysis High Mobility Group Proteins Histones/isolation & purification Nucleosomes/ultrastructure

Chemicals

Chromosomal Proteins, Non-Histone High Mobility Group Proteins Histones Nucleosomes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Albanese I

Weintraub H

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.12.2787

Published

1980-06-25

Pages

2787-805

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324121

Subset

External Links

PubMed source DOI: 10.1093/nar/8.12.2787 PMC Full Text View journal details

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Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes.

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