Partial purification of transcriptionally active nucleosomes from trout testis cells.

Nucleic acids research ·Vol. 5 ·No. 11 ·1978-11-00 ·Pages 4155-63

Levy B, Dixon GH

Abstract

Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.

MeSH Terms

Animals Cell Fractionation Cell Nucleus/metabolism,ultrastructure Chromatin/metabolism,ultrastructure Chromosomal Proteins, Non-Histone/isolation & purification DNA/isolation & purification Female Histones/isolation & purification Kinetics Male Nucleic Acid Hybridization Testis/metabolism Transcription, Genetic Trout

Chemicals

Chromatin Chromosomal Proteins, Non-Histone Histones DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Levy B

Dixon G H

References (12)

12 references, click to expand

Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
Distribution of messenger RNA-coding sequences in fractionated chromatin.
Cell. 1977 Dec;12(4):953-62 PMID: 597865
Enrichment for the globin coding region in a chromatin fraction from chick reticulocytes by endonuclease digestion.
Gene. 1977;2(3-4):147-58 PMID: 344138
Chromatin.
Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
Structure of chromatin.
Annu Rev Biochem. 1977;46:931-54 PMID: 332067
Partial purification of the template-active fraction of chromatin: a preliminary report.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2193-7 PMID: 4526205
Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810-4 PMID: 268631
Structure of transcriptionally-active chromatin subunits.
Nucleic Acids Res. 1977 Sep;4(9):3155-73 PMID: 909802
Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.
Nucleic Acids Res. 1977 Apr;4(4):883-98 PMID: 866196
Changes in the sequence diversity of polyadenylated cytoplasmic RNA during testis differentiation in rainbow trout (Salmo gairdnerii).
Eur J Biochem. 1977 Mar 15;74(1):61-7 PMID: 856575
Chromosomal proteins and chromatin structure.
Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
Methods for fractionation of chromatin into transcriptionally active and inactive segments.
Methods Cell Biol. 1977;16:421-36 PMID: 329060

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.11.4155

Published

1978-11-00

Pages

4155-63

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342740

Subset

External Links

PubMed source DOI: 10.1093/nar/5.11.4155 PMC Full Text View journal details

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Partial purification of transcriptionally active nucleosomes from trout testis cells.

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