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PMID: 493146 Published · ppublish English Journal Article

On the de novo formation of compact oligonucleosomes at high ionic strength. Evidence for nucleosomal sliding in high salt.

Nucleic acids research ·Vol. 7 ·No. 2 ·1979-09-25 ·Pages 291-304

Weischet WO

Abstract

Histone H 1-depleted chromatin made from acid extracted, intact nuclei was exposed to various ionic strengths. NaCl concentrations above 0.3 M sufficed to generate novel oligonucleosomes formerly characterized as "compact oligomers" and "spacerless dinucleosomes". Such particles could not be identified within H 1-depleted nuclei or chromatin at low ionic strengths. Their formation, proceeding within days at 0 degrees C, was accelerated by increasing ionic strengths. The data was discussed in terms of a salt-induced motion of nucleosomal core particles along the DNA to form compact oligomers.

MeSH Terms
Animals Cattle Chromatin/ultrastructure Histones Kinetics Nucleic Acid Conformation Nucleosomes/ultrastructure Oligodeoxyribonucleotides Oligonucleotides Osmolar Concentration Sodium Chloride Thymus Gland/analysis
Chemicals
Chromatin Histones Nucleosomes Oligodeoxyribonucleotides Oligonucleotides Sodium Chloride
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Weischet W O
References (24)
24 references, click to expand
  1. Nucleosome conformation: pH and organic solvent effects.
    Nucleic Acids Res. 1978 Oct;5(10):3881-97 PMID: 31605
  2. Stability of nucleosomes in native and reconstituted chromatins.
    Nucleic Acids Res. 1976 Nov;3(11):3173-92 PMID: 188018
  3. On the occurrence of nucleosome phasing in chromatin.
    Cell. 1977 Nov;12(3):829-36 PMID: 336221
  4. Quantitative analysis of the digestion of yeast chromatin by staphylococcal nuclease.
    Biochemistry. 1977 Feb 8;16(3):463-71 PMID: 319824
  5. Compact oligomers and nucleosome phasing.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3583-7 PMID: 278974
  6. H1 histone and the condensation of chromatin and DNA.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:253-63 PMID: 277344
  7. Electric dichroism studies of the size and shape of nucleosomal particles.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:207-14 PMID: 277341
  8. The structure of the chromatin core particle in solution.
    Nucleic Acids Res. 1977 Sep;4(9):3199-214 PMID: 561952
  9. The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease.
    Nucleic Acids Res. 1979;6(5):1843-62 PMID: 450715
  10. An octamer of core histones in solution: central role of the H3-H4 tetramer in the self-assembly.
    Biochemistry. 1979 Mar 6;18(5):760-8 PMID: 420814
  11. The histone core complex: an octamer assembled by two sets of protein-protein interactions.
    Biochemistry. 1978 Nov 14;17(23):4955-64 PMID: 718868
  12. The interaction of core histones with DNA: equilibrium binding studies.
    Nucleic Acids Res. 1978 Oct;5(10):3643-63 PMID: 724497
  13. Effect of histone acetylation on structure and in vitro transcription of chromatin.
    Nucleic Acids Res. 1978 Oct;5(10):3523-47 PMID: 724494
  14. Reconstitution of chromatin: assembly of the nucleosome.
    Nucleic Acids Res. 1978 Feb;5(2):505-21 PMID: 634796
  15. Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin.
    Eur J Biochem. 1978 Feb;83(2):615-28 PMID: 631138
  16. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.
    Nucleic Acids Res. 1977 Dec;4(12):4077-89 PMID: 600791
  17. Reconstitution of chromatin core particles.
    Biochemistry. 1977 Nov 29;16(24):5295-303 PMID: 921932
  18. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  19. Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation.
    Anal Biochem. 1974 Sep;61(1):165-83 PMID: 4413794
  20. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  21. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  22. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  23. A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH.
    Exp Cell Res. 1970 Mar;59(3):469-78 PMID: 5461579
  24. A model for particulate structure in chromatin.
    Nucleic Acids Res. 1974 Nov;1(11):1579-86 PMID: 10793713
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.2.291
Published
1979-09-25
Pages
291-304
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328018
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.2.291 PMC Full Text View journal details
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