The interaction of core histones with DNA: equilibrium binding studies.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3643-63

Burton DR, Butler MJ, Hyde JE, Phillips D, Skidmore CJ, Walker IO

Abstract

The binding of core histone proteins to DNA, measured as a function of [NaCl[ is a reversible process. Dissociation and reassociation occurs in two stages. Between 0.7 and 1.2 M NaCl H2a H2b bind non-cooperatively as an equimolar complex with deltaGo = 1.6 Kcals/mole at 4 degree C and 1.0 M NaCl. Between 1.2 and 2.0 M NaCl H3 and H4 bind cooperatively as an equimolar complex with delta Go = 7.4 Kcal/mole at 4 degree C and 1.0 M NaCl. The proper binding of H2a and H2b requires the presence of bound H3 and H4. Nuclease digestion of the H3-H4 DNA produces a tetramer of H3-H4 bound to fragments of DNA 145, 125 and 104 base pairs long. Thus an H3-H4 tetramer can protect fragments of DNA as long as those found in complete core particles and must therefore span the nucleosome core particle.

MeSH Terms

Animals Cattle Cell-Free System Chromatin/ultrastructure Circular Dichroism DNA/metabolism Dose-Response Relationship, Drug Histones/metabolism Micrococcal Nuclease/metabolism Protein Binding/drug effects Sodium Chloride/pharmacology Thymus Gland

Chemicals

Chromatin Histones Sodium Chloride DNA Micrococcal Nuclease

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Burton D R

Butler M J

Hyde J E

Phillips D

Skidmore C J

Walker I O

References (18)

18 references, click to expand

Specific folding and contraction of DNA by histones H3 and H4.
Cell. 1977 Jul;11(3):609-18 PMID: 195743
Preparation of native chromatin and damage caused by shearing.
Science. 1975 Mar 28;187(4182):1203-6 PMID: 17754290
Chromatin.
Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
Higher order coiling of DNA in chromatin.
Cell. 1977 Sep;12(1):83-100 PMID: 409504
Characterization of the octamer of histones free in solution.
J Mol Biol. 1977 Nov;116(4):769-81 PMID: 592400
The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
Cell. 1976 Jul;8(3):333-47 PMID: 986252
Chromatin structure as probed by nucleases and proteases: evidence for the central role of histones H3 and H4.
Cell. 1976 Sep;9(1):179-93 PMID: 987855
A model for chromatin based upon two symmetrically paired half-nucleosomes.
Cell. 1976 Nov;9(3):409-17 PMID: 991271
Chromosomal proteins and chromatin structure.
Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
Covalent cross-linking of histones in chromatin.
FEBS Lett. 1975 Feb 1;50(2):150-4 PMID: 1112409
Binding of flexible ligands to macromolecules.
Nature. 1975 Feb 27;253(5494):753-5 PMID: 1113874
Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.
Biochemistry. 1975 Jul;14(13):2921-5 PMID: 1148185
Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt.
Cell. 1975 Sep;6(1):85-110 PMID: 1164735
An investigation of the conformational and self-aggregational processes of histones using 1H and 13C nuclear magnetic resonance.
Biochemistry. 1975 Oct 21;14(21):4590-600 PMID: 1182105
The structure of partially histone depleted nucleohistone.
FEBS Lett. 1973 May 15;32(1):175-8 PMID: 4715681
[NaCl dissociation of the nucleoproteins of chicken erythrocytes].
Eur J Biochem. 1969 Aug;10(1):102-9 PMID: 5345975
The partial dissociation of nucleohistone by salts. Circular dichroism and denaturation studies.
Eur J Biochem. 1970 Nov;16(3):524-31 PMID: 5477298
Structure of chromatin.
Annu Rev Biochem. 1977;46:931-54 PMID: 332067

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.10.3643

Published

1978-10-00

Pages

3643-63

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342701

Subset

External Links

PubMed source DOI: 10.1093/nar/5.10.3643 PMC Full Text View journal details

Analysis Services

The interaction of core histones with DNA: equilibrium binding studies.

Contact

WeChat Official Account