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PMID: 634796 Published · ppublish English Journal Article

Reconstitution of chromatin: assembly of the nucleosome.

Nucleic acids research ·Vol. 5 ·No. 2 ·1978-02-00 ·Pages 505-21

Wilhelm FX, Wilhelm ML, Erard M, Duane MP

Abstract

The order of reassociation of the four histones H2a, H2b, H3 and H4 to the DNA during the reconstitution of chromatin was determined. At each step of the reconstitution the DNA and associated histones were separated from the free histones by centrifugation in a glycerol gradient. The unbound and reassociated histones were analysed by gel electrophoresis and the histone-DNA complexes characterized by circular dichroism and electron microscopy. We show that H3 and H4 bind first to the DNA between 1.2 M NaCl and 0.85 M NaCl and impose a nucleosome like structure; in a second step histones H2a and H2b are placed around this kernel to complete the nucleosome.

MeSH Terms
Animals Chickens Chromatin/ultrastructure Circular Dichroism DNA/metabolism Erythrocytes Histones/metabolism Microscopy, Electron Osmolar Concentration Protein Binding Sodium Chloride
Chemicals
Chromatin Histones Sodium Chloride DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wilhelm F X
Wilhelm M L
Erard M
Duane M P
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.2.505
Published
1978-02-00
Pages
505-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341998
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.2.505 PMC Full Text View journal details
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