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PMID: 995642 Published · ppublish English Journal Article

Circular dichroism as a probe of DNA structure inside reconstituted nucleohistones.

Nucleic acids research ·Vol. 3 ·No. 10 ·1976-10-00 ·Pages 2507-19

Garel A, Kovacs AM, Champagne M, Daune M

Abstract

Reconstituted nucleohistones were obtained by mixing in given conditions acid extracted histones and eukaryotic DNA. The histone/DNA ratio (w/w) was in the range 0.35 - 0.95. With the four histones (H2A2B) we have been able to obtain subunits (nucleosomes or upsilon-bodies). The variation of cirsular dichroism signal with temperature at 280 nm was measured to follow structural changes of the DNA inside the complex. The true change of ellipticity (see article) of histone-bound DNA regions, is similar for reconstituted nucleohistone and H1-depleted chromatin, and is therefore a physical probe of the presence of nucleosomes.

MeSH Terms
Animals Chickens Chromatin/ultrastructure Circular Dichroism DNA Histones Nucleic Acid Conformation Nucleic Acid Denaturation Nucleoproteins Structure-Activity Relationship
Chemicals
Chromatin Histones Nucleoproteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garel A
Kovacs A M
Champagne M
Daune M
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.10.2507
Published
1976-10-00
Pages
2507-19
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343109
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.10.2507 PMC Full Text View journal details
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