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PMID: 6757869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional analysis of separated strands of killer double-stranded RNA of yeast.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6903-18

Thiele DJ, Leibowitz MJ

Abstract

The two strands of the M double-stranded RNA species from a killer strain of Saccharomyces cerevisiae have been separated, and the 3'-terminal sequences of these strands have been determined. The positive strand programs the synthesis of the putative killer toxin precursor (M-p32) in a rabbit reticulocyte in vitro translation system. Only the negative strand hybridizes to the positive polarity transcript (m) synthesized in vitro by the virion-associated transcriptase activity. Secondary structural analysis of the extreme 3'-terminus of the negative strand using S1 nuclease is consistent with the presence of a large stem and loop structure previously proposed on the basis of RNA sequence data. This structure, and a similar structure at the corresponding 5'-terminus of the positive strand, may have functional significance in vivo.

MeSH Terms
Animals Base Sequence Mutation Mycotoxins/genetics Nucleic Acid Conformation Nucleic Acid Hybridization Protein Biosynthesis RNA, Double-Stranded/genetics,isolation & purification Rabbits Reticulocytes/metabolism Saccharomyces cerevisiae/genetics
Chemicals
Mycotoxins RNA, Double-Stranded
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thiele D J
Leibowitz M J
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.21.6903
Published
1982-11-11
Pages
6903-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326973
Subset
IM
Grants
NIAID NIH HHS · AI-14843 · United States
NCI NIH HHS · CA-09069 · United States
Databases
GENBANK
M12538
External Links
PubMed source DOI: 10.1093/nar/10.21.6903 PMC Full Text View journal details
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