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PMID: 7029463 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequences at the 3' ends of yeast viral dsRNAs: proposed transcriptase and replicase initiation sites.

Nucleic acids research ·Vol. 9 ·No. 16 ·1981-08-25 ·Pages 4007-21

Brennan VE, Field L, Cizdziel P, Bruenn JA

Abstract

ScV is a double-stranded RNA virus of yeast consisting of two separately encapsidated dsRNAs (L and M). ScV-1 and ScV-2 are two dsRNA viruses present in two different yeast killer strains, K1 and K2. Our 3' end sequence analysis shows that the two sets of viral dsRNAs from ScV-1 and ScV-2 are very similar. Consensus sequences for transcriptase and replicase initiation are proposed. A stem and loop structure with a 3' terminal AUGC sequence, like that of several plant virus plus strand RNAs, is present at the putative replicase initiation site of one of the yeast viral RNA plus strands.

MeSH Terms
Base Sequence Binding Sites DNA-Directed RNA Polymerases/metabolism Nucleic Acid Conformation RNA Nucleotidyltransferases/metabolism RNA Viruses/metabolism RNA, Double-Stranded/metabolism RNA, Viral/metabolism RNA-Dependent RNA Polymerase/metabolism Saccharomyces cerevisiae
Chemicals
RNA, Double-Stranded RNA, Viral RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brennan V E
Field L
Cizdziel P
Bruenn J A
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.16.4007
Published
1981-08-25
Pages
4007-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327411
Subset
IM
Grants
NIGMS NIH HHS · GM22200 · United States
External Links
PubMed source DOI: 10.1093/nar/9.16.4007 PMC Full Text View journal details
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