Home LiteratureArticle Details
PMID: 6579530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 21 ·1983-11-00 ·Pages 6446-50

Tamanoi F, Stillman BW

Abstract

Initiation of adenovirus DNA replication in vitro occurs on a linearized plasmid DNA containing 3,327 base pairs of the adenovirus terminal sequence. Various deletions have been constructed in the plasmid DNA and their template activities examined. Deletions from an internal restriction enzyme cleavage site that retain only 20 base pairs or more of the adenovirus terminal sequence support initiation and limited chain elongation, whereas deletions that leave 14 base pairs or less of the terminal sequence do not. On the other hand, all deletions extending from the very terminus of the adenovirus DNA destroy the template activity. The terminal 20 base pairs of adenovirus DNA contain a sequence A-T-A-A-T-A-T-A-C-C, which is perfectly conserved in the DNAs from different serotypes of human adenovirus. Base changes within the conserved sequence greatly reduce the template activity. These results suggest that the terminal 20 base pairs constitute a functional origin for the initiation of adenovirus DNA replication in vitro.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Chromosome Deletion DNA Replication Plasmids Templates, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tamanoi F
Stillman B W
References (49)
49 references, click to expand
  1. A circula DNA-protein complex from adenoviruses.
    Virology. 1973 Nov;56(1):54-69 PMID: 4745628
  2. Adenoviral DNA replication: DNA sequences and enzymes required for initiation in vitro.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:741-50 PMID: 6574870
  3. The nucleotide sequence at the termini of adenovirus type 5 DNA.
    Nucleic Acids Res. 1977 Dec;4(12):4371-89 PMID: 600799
  4. Construction of a viable SV40 variant containing two functional origins of DNA replication.
    Cell. 1978 Apr;13(4):791-8 PMID: 207442
  5. Adenovirus DNA: structure and function of a novel replicon.
    Cell. 1978 Aug;14(4):761-73 PMID: 357012
  6. Adenovirus DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):655-9 PMID: 284391
  7. An adenovirus protein associated with the ends of replicating DNA molecules.
    Virology. 1979 Feb;93(1):69-79 PMID: 433158
  8. The nucleotide sequences at the termini of adenovirus-2 DNA.
    J Mol Biol. 1979 Mar 15;128(4):577-94 PMID: 318164
  9. Nucleotide sequence at the inverted terminal repetition of adenovirus type 2 DNA.
    Biochem Biophys Res Commun. 1979 Apr 13;87(3):671-8 PMID: 454416
  10. Sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between SA7 termini and SV40 DNA.
    Cell. 1979 Jul;17(3):705-13 PMID: 225041
  11. Dissection and comparative anatomy of the origins of replication of lambdoid phages.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:155-63 PMID: 157834
  12. Functional analysis of the replicator structure of lambdoid bacteriophage DNAs.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:165-78 PMID: 157835
  13. Single-stranded phage replication: positive- and negative-strand DNA synthesis.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:349-65 PMID: 157839
  14. Presence of protein at the termini of intracellular adenovirus type 5 DNA.
    Biochim Biophys Acta. 1979 Jun 20;563(1):89-99 PMID: 227463
  15. Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication.
    J Mol Biol. 1979 Jul 15;131(4):801-17 PMID: 229233
  16. The genome of human papovavirus BKV.
    Cell. 1979 Dec;18(4):963-77 PMID: 229976
  17. The inverted terminal repetition of the DNA of weakly oncogenic adenovirus type 7.
    Gene. 1979 Dec;8(1):7-15 PMID: 231543
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. Cold-sensitive regulatory mutants of simian virus 40.
    J Mol Biol. 1980 Jun 15;140(1):129-42 PMID: 6251230
  20. Structure and gene organization in the transformed Hind III-G fragment of Ad12.
    Cell. 1980 Jul;20(3):777-86 PMID: 6251973
  21. Comparative sequence analysis of the inverted terminal repetitions from different adenoviruses.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3831-5 PMID: 6253991
  22. Expression of early genes of origin-defective mutants of simian virus 40.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3898-902 PMID: 6254000
  23. Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5105-9 PMID: 6933548
  24. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5 PMID: 6256739
  25. Replication of mouse adenovirus strain FL DNA.
    Virology. 1981 Feb;109(1):1-12 PMID: 6927844
  26. Identification of the gene and mRNA for the adenovirus terminal protein precursor.
    Cell. 1981 Feb;23(2):497-508 PMID: 7471210
  27. Adenovirus DNA replication in vitro: a protein linked to the 5' end of nascent DNA strands.
    J Virol. 1981 Jan;37(1):139-47 PMID: 7218423
  28. Nucleotide sequences at the termini of phi 29 DNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1336-40 PMID: 6262789
  29. Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1446-50 PMID: 6262800
  30. Processing of the adenovirus terminal protein.
    J Virol. 1981 Apr;38(1):272-7 PMID: 7241655
  31. Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin.
    Nucleic Acids Res. 1981 Apr 24;9(8):1789-99 PMID: 6264392
  32. Formation of a covalent complex between the 80,000-dalton adenovirus terminal protein and 5'-dCMP in vitro.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2678-82 PMID: 6942401
  33. Characterization of the effect of aphidicolin on adenovirus DNA replication: evidence in support of a protein primer model of initiation.
    Nucleic Acids Res. 1981 Oct 10;9(19):4919-38 PMID: 6796938
  34. Adenovirus DNA replication in vitro: purification of the terminal protein in a functional form.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6779-83 PMID: 6947251
  35. Territorial limits and functional anatomy of the simian virus 40 replication origin.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5 PMID: 6281769
  36. Adenoviral protein-primed initiation of DNA chains in vitro.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2442-6 PMID: 6283524
  37. Function of adenovirus terminal protein in the initiation of DNA replication.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2221-5 PMID: 6954537
  38. Structural characterization of the adenovirus 18 inverted terminal repetition.
    Virology. 1982 Sep;121(2):230-9 PMID: 6289515
  39. A common sequence in the inverted terminal repetitions of human and avian adenoviruses.
    Gene. 1982 May;18(2):193-7 PMID: 6290323
  40. Physical mapping of the genome and sequence analysis at the inverted terminal repetition of adenovirus type 4 DNA.
    Gene. 1982 Jun;18(3):329-34 PMID: 6290336
  41. Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5225-9 PMID: 6957861
  42. Conserved sequences at the origin of adenovirus DNA replication.
    J Virol. 1982 Nov;44(2):530-7 PMID: 7143575
  43. Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438-42 PMID: 6216480
  44. Effects of the adenovirus H5ts125 and H5ts107 DNA binding proteins on DNA replication in vitro.
    Virology. 1983 Jan 30;124(2):380-9 PMID: 6297162
  45. Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replication.
    Cell. 1982 Dec;31(3 Pt 2):613-23 PMID: 6297776
  46. Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein.
    Nucleic Acids Res. 1983 Apr 11;11(7):1975-89 PMID: 6300787
  47. Evidence for an altered adenovirus DNA polymerase in cells infected with the mutant H5ts149.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1589-93 PMID: 6572922
  48. In vitro complementation as an assay for purification of adenovirus DNA replication proteins.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):935-9 PMID: 6573663
  49. Identification of a protein linked to the ends of adenovirus DNA.
    Cell. 1977 Jun;11(2):283-95 PMID: 196758
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.21.6446
Published
1983-11-00
Pages
6446-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390130
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.21.6446 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2