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PMID: 6500568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The association of the nucleolus and the short arm of acrocentric chromosomes with the XY pair in human spermatocytes: its possible role in facilitating sex-chromosome acrocentric translocations.

Human genetics ·Vol. 68 ·No. 2 ·1984-00-00 ·Pages 173-80

Stahl A, Hartung M, Devictor M, Bergé-Lefranc JL

Abstract

Sex vesicle-nucleolus association was observed in 12% of zygotene and pachytene human spermatocytes using Giemsa and NOR-silver stained preparations. The silver-positive area of the nucleolus, corresponding to the nucleolus organizer (NOR), was usually close to the XY pair. C-banding frequently showed the terminal chromomere, formed by the condensed short arm of an acrocentric bivalent, attached to the sex vesicle. When a nucleolus produced by transcription of rDNA was connected to the short arm, it seemed to be secondarily associated with the sex vesicle. Non-transcribed ribosomal genes, which did not form a nucleolus, were revealed by in situ hybridization. Autoradiographs showed the rDNA-containing short arm of acrocentric bivalents associated with the sex vesicle in 18% of spermatocytes. The difference with the frequency of nucleolus-XY pair association was partially explained by the presence of inactive ribosomal genes. Moreover, electron microscopy showed that the dimensions of the newly formed nucleoli at early zygotene did not exceed 0.5 micron; they can be missed in light microscope investigations. From early zygotene to late pachytene, close relationships were observed between the sex vesicle chromatin and that of the associated acrocentric bivalent, especially in the short arm region. These relationships might explain the frequent involvement of acrocentrics in Y-autosome and X-autosome translocations occurring during male meiosis.

MeSH Terms
Cell Nucleolus/ultrastructure Chromosome Banding Humans Male Meiosis Microscopy, Electron Nucleolus Organizer Region Spermatogenesis Translocation, Genetic Y Chromosome
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stahl A
Hartung M
Devictor M
Bergé-Lefranc J L
References (47)
47 references, click to expand
  1. Identification of pachytene bivalents in human male meiosis using G-banding technique.
    Chromosoma. 1975 Oct 14;52(3):275-82 PMID: 1183301
  2. Eukaryotic DNA methylation.
    Hum Genet. 1983;64(4):315-33 PMID: 6194100
  3. Variation in ribosomal RNA gene number in mouse chromosomes.
    Cytogenet Cell Genet. 1976;17(6):307-16 PMID: 17494622
  4. The localization of nucleic acids and the argentaffin substance in the sex vesicle of mouse spermatocytes.
    Exp Cell Res. 1967 Aug;47(1):86-96 PMID: 6068436
  5. Sex-linkage and the sex chromosomes in man.
    Ann Eugen. 1954 Jan;18(3):255-61 PMID: 13125206
  6. Chromosome assignment of two cloned DNA probes hybridizing predominantly to human sex chromosomes.
    Hum Genet. 1984;65(3):257-61 PMID: 6538180
  7. Down's syndrome in the male. Reproductive pathology and meiotic studies.
    Hum Genet. 1983;63(2):132-8 PMID: 6220959
  8. THE FINE STRUCTURE OF THE MAMMALIAN CHROMOSOME IN MEIOTIC PROPHASE WITH SPECIAL REFERENCE TO THE SYNAPTINEMAL COMPLEX.
    J Anat. 1964 Apr;98:163-73 PMID: 14154419
  9. The behavior of the XY pair in mammals.
    Int Rev Cytol. 1974;38(0):273-317 PMID: 4854664
  10. A direct demonstration of somatically paired heterochromatin of human chromosomes.
    Cytogenet Cell Genet. 1983;36(3):554-61 PMID: 6196158
  11. Evolution of a human Y chromosome-specific repeated sequence.
    Cell. 1978 Mar;13(3):453-60 PMID: 657266
  12. Structural basis for Robertsonian translocations in man: association of ribosomal genes in the nucleolar fibrillar center in meiotic spermatocytes and oocytes.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):5946-50 PMID: 6577464
  13. Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function.
    Cytogenet Cell Genet. 1981;31(2):47-57 PMID: 7307582
  14. Satellite DNA and heterochromatin variants: the case for unequal mitotic crossing over.
    Hum Genet. 1979 Mar 12;47(2):169-86 PMID: 374224
  15. Report of the Committee on the Genetic Constitution of the X and Y Chromosomes.
    Cytogenet Cell Genet. 1984;37(1-4):176-204 PMID: 6360558
  16. An air-drying method for first meiotic prophase preparations from mammalian ovaries.
    J Reprod Fertil. 1974 Feb;36(2):409-11 PMID: 4594619
  17. THE SITES OF NUCLEOLUS FORMATION IN HUMAN PACHYTENE CHROMOSOMES.
    Cytogenetics. 1964;3:124-34 PMID: 14190611
  18. Nonhomologous associations of C-heterochromatin at human male meitoic prophase.
    Cytogenet Cell Genet. 1979;23(1-2):23-32 PMID: 761482
  19. 5-Methylcytosine in heterochromatic regions of chromosomes: chimpanzee and gorilla compared to the human.
    Chromosoma. 1975 Sep 15;52(1):59-66 PMID: 1175458
  20. X-autosome translocations: cytogenetic characteristics and their consequences.
    Hum Genet. 1982;61(4):295-309 PMID: 7152515
  21. The fate of DNA satellites I, II, III and ribosomal DNA in a familial dicentric chromosome 13:14.
    Hum Genet. 1978 Mar 17;41(2):131-41 PMID: 640652
  22. [Meiotic chromosomes of humans. II. The nucleolus. The chiasmata. 3. Male sterility].
    Ann Genet. 1970 Sep;13(3):169-82 PMID: 5313144
  23. Localisation of a male-specific DNA fragment to a sub-region of the human Y chromosome.
    Nature. 1978 Mar 23;272(5651):324-8 PMID: 76288
  24. Ultrastructural localization of Ag-NOR stained proteins in the nucleolus during the cell cycle and in other nucleolar structures.
    Chromosoma. 1980;79(3):349-62 PMID: 6156811
  25. Identifying Y chromosome in interphase nuclei of the human brain.
    Experientia. 1973 Nov 15;29(11):1428-9 PMID: 4761264
  26. Frequency of "Y chromatin body" in human skin fibroblasts in tissue culture, and its relation to growth phase.
    Exp Cell Res. 1971 Apr;65(2):473-5 PMID: 5554043
  27. The fine structure of the sex vesicle and sex chromosome association in spermatocytes of mouse, golden hamster and field vole.
    J Anat. 1966 Oct;100(Pt 4):787-99 PMID: 5972764
  28. The chromosomal distribution of human satellite III DNA during meiosis.
    Cytobios. 1976;15(58-59):79-84 PMID: 1001023
  29. Interphase association of human "Y body" with nucleolus.
    Johns Hopkins Med J. 1972 Apr;130(4):254-8 PMID: 4110614
  30. The location of four human satellite DNAs on human chromosomes.
    Exp Cell Res. 1975 Apr;92(1):148-58 PMID: 48464
  31. Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.
    Chromosoma. 1975 Nov 20;53(1):37-50 PMID: 53131
  32. Nucleolar and perichromosomal RNA synthesis during meiotic prophase in the mouse testis.
    J Cell Biol. 1974 Jan;60(1):39-53 PMID: 4203361
  33. Quinacrine studies of sex chromatin and nucleoli in human brain.
    Humangenetik. 1973 Dec 20;20(4):329-33 PMID: 4768109
  34. An infertile male with balanced Y;19 translocation. Review of Y;autosome translocations.
    Ann Genet. 1979;22(4):189-194 PMID: 399165
  35. Synaptosomal complexes and associated structures in microspread human spermatocytes.
    Chromosoma. 1980;81(3):315-37 PMID: 7192619
  36. 5-Methylcytosine localised in mammalian constitutive heterochromatin.
    Nature. 1974 Oct 18;251(5476):636-7 PMID: 4609195
  37. Nucleolar RNA synthesis of meiotic prophase spermatocytes in the human testis.
    Chromosoma. 1975 Nov 24;53(2):141-51 PMID: 1201687
  38. Attraction between centric heterochromatin of human chromosomes.
    Cytogenet Cell Genet. 1975;15(2):66-80 PMID: 1183239
  39. Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining.
    Chromosoma. 1979 Feb 21;71(2):197-216 PMID: 85513
  40. The three-dimensional reconstruction of the XY chromosomal pair in human spermatocytes.
    J Cell Biol. 1970 Apr;45(1):43-53 PMID: 5458999
  41. Heterochromatic regions and nucleolus organizers in chromosomes of the mouse, Mus musculus.
    Exp Cell Res. 1957 Oct;13(2):358-64 PMID: 13480303
  42. Satellite DNA loss and nucleolar organiser activity in an individual with a de novo chromosome 13,14 translocation.
    Clin Genet. 1979 Jun;15(6):518-29 PMID: 466852
  43. Synaptonemal complex complement of man in spreads of spermatocytes, with details of the sex chromosome pair.
    Science. 1975 Jan 31;187(4174):363-5 PMID: 1111110
  44. Theiler's virus persists in glial cells during demyelinating disease.
    Cell. 1981 Oct;26(1 Pt 1):123-8 PMID: 6276007
  45. Para-nucleolar position of the human Y chromosome in interphase nuclei.
    Nature. 1971 Aug 20;232(5312):556-7 PMID: 4937496
  46. Localisation of male determining factors in man: a thorough review of structural anomalies of the Y chromosome.
    J Med Genet. 1981 Jun;18(3):161-95 PMID: 7017147
  47. EM autoradiographic evidence that DNA synthesis occurs at recombination nodules during meiosis in Drosophila melanogaster females.
    Chromosoma. 1981;83(1):59-80 PMID: 6790251
Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00279310
Published
1984-00-00
Pages
173-80
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00279310 View journal details
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