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PMID: 6448990 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial inhibition of histone deacetylase in active chromatin by HMG 14 and HMG 17.

Nucleic acids research ·Vol. 8 ·No. 9 ·1980-05-10 ·Pages 1947-63

Reeves R, Candido EP

Abstract

Digestion of isolated Friend erythroleukemic cell nuclei with DNase I under conditions which selectively destroy the DNA of transcriptionally "active" genes releases into the supernatant fraction proteins of the non-histone "High Mobility Group" (HMGs). Two of these, HMG-14 and HMG-17(identified by solubility in trichloroacetic acid, electrophoretic mobility on SDS-polyacrylamide gels and by amino acid composition) will partially inhibit the endogenous mouse cell histone deacetylase enzymes when added to in vitro assay mixtures. Other closely related proteins do not share this inhibitory ability and thus the reaction with the enzymes appears to be specific. Since these two HMG proteins appear to be preferentially associated with the "active" fraction of chromatin, these findings have important implications for possible models of eukaryotic gene regulatory mechanisms.

MeSH Terms
Amidohydrolases/antagonists & inhibitors Animals Cell Line Chromatin/metabolism Chromosomal Proteins, Non-Histone/metabolism,pharmacology Friend murine leukemia virus High Mobility Group Proteins Histone Deacetylase Inhibitors In Vitro Techniques Leukemia, Experimental/metabolism Mice Neoplasm Proteins/pharmacology Nucleosomes/metabolism Tumor Virus Infections/metabolism
Chemicals
Chromatin Chromosomal Proteins, Non-Histone High Mobility Group Proteins Histone Deacetylase Inhibitors Neoplasm Proteins Nucleosomes Amidohydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reeves R
Candido E P
References (40)
40 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. A new group of chromatin-associated proteins with a high content of acidic and basic amino acids.
    Eur J Biochem. 1973 Sep 21;38(1):14-9 PMID: 4774120
  3. Interactions of a purified non-histone chromosomal protein with DNA and histone.
    Eur J Biochem. 1974 Sep 1;47(2):263-70 PMID: 4472153
  4. Interaction of a non-histone chromatin protein (high-mobility group protein 2) with DNA.
    Eur J Biochem. 1975 Jun;54(2):427-33 PMID: 1175594
  5. Processing of newly synthesized histone molecules.
    Science. 1975 Oct 10;190(4210):117-28 PMID: 1166303
  6. An improved large scale fractionation of high mobility group non-histone chromatin proteins.
    Biochim Biophys Acta. 1975 Oct 20;405(2):280-91 PMID: 1180957
  7. Heterogeneity of chromatin subunits in vitro and location of histone H1.
    Nucleic Acids Res. 1976 Feb;3(2):477-92 PMID: 1257057
  8. Sequence composition of the template-active fraction of rat liver chromatin.
    Biochemistry. 1976 Jun 1;15(11):2472-83 PMID: 179568
  9. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  11. A class of chromatin particles associated with nonhistone proteins.
    Biochemistry. 1976 Aug 10;15(16):3510-5 PMID: 952874
  12. The presence of high mobility group non-histone chromatin proteins in isolated nucleosomes.
    FEBS Lett. 1977 Jan 15;73(1):85-8 PMID: 838051
  13. Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.
    Nucleic Acids Res. 1977 Apr;4(4):883-98 PMID: 866196
  14. Nucleosomes and subnucleosomes: heterogeneity and composition.
    Cell. 1977 Jul;11(3):619-29 PMID: 884737
  15. The primary structure of a non-histone chromosomal protein.
    Eur J Biochem. 1977 Jun 15;76(2):461-8 PMID: 330164
  16. Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810-4 PMID: 268631
  17. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  18. Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin.
    Cell. 1977 Oct;12(2):409-15 PMID: 562236
  19. Immunological relatedness of high mobility group chromosomal proteins from calf thymus.
    J Biol Chem. 1978 Mar 10;253(5):1694-9 PMID: 75209
  20. Structure of chromatin containing extensively acetylated H3 and H4.
    Cell. 1978 Apr;13(4):691-9 PMID: 657272
  21. A study of the localization of high mobility group proteins in chromatin.
    Can J Biochem. 1978 Jun;56(6):480-91 PMID: 667694
  22. Sodium butyrate inhibits histone deacetylation in cultured cells.
    Cell. 1978 May;14(1):105-13 PMID: 667927
  23. Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43 PMID: 276864
  24. Acetylated histone H4 is preferentially associated with template-active chromatin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3574-7 PMID: 278972
  25. Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion.
    Cell. 1978 Sep;15(1):141-50 PMID: 699037
  26. Non-histone proteins in mononucleosomes and subnucleosomes.
    Eur J Biochem. 1978 Nov 2;91(1):291-301 PMID: 720344
  27. Partial purification of transcriptionally active nucleosomes from trout testis cells.
    Nucleic Acids Res. 1978 Nov;5(11):4155-63 PMID: 569293
  28. A subset of trout testis nucleosomes enriched in transcribed DNA sequences contains high mobility group proteins as major structural components.
    J Biol Chem. 1979 Feb 10;254(3):609-20 PMID: 762085
  29. Multiacetylated forms of H4 are found in a putative transcriptionally competent chromatin fraction from trout testis.
    Nucleic Acids Res. 1979 Jan;6(1):259-74 PMID: 424292
  30. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 PMID: 284387
  31. Sodium butyrate induces new gene expression in Friend erythroleukemic cells.
    J Biol Chem. 1979 May 25;254(10):4283-90 PMID: 285931
  32. Comparison of non histone proteins selectively associated with nucleosomes with proteins released during limited DNase digestions.
    Nucleic Acids Res. 1979 Mar;6(3):953-66 PMID: 440975
  33. Limited action of micrococcal nuclease on trout testis nuclei generates two mononucleosome subsets enriched in transcribed DNA sequences.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1682-6 PMID: 287007
  34. The complete amino-acid sequence of a trout-testis non-histone protein, H6, localized in a subset of nucleosomes and its similarity to calf-thymus non-histone proteins HMG-14 and HMG-17.
    Eur J Biochem. 1979 Mar 15;95(1):193-202 PMID: 456349
  35. The primary structure of the nucleosome-associated chromosomal protein HMG 14.
    FEBS Lett. 1979 Apr 15;100(2):394-8 PMID: 456580
  36. Histones.
    Annu Rev Biochem. 1979;48:159-91 PMID: 382983
  37. Chromatin fractionation procedure that yields nucleosomes containing near-stoichiometric amounts of high mobility group nonhistone chromosomal proteins.
    Biochemistry. 1979 Aug 21;18(17):3739-48 PMID: 476083
  38. Effect of DNase I on histone acetyltransferase and deacetylase enzyme activities in Friend cell nuclei: evidence for a putative inhibitor of the deacetylase enzymes in transcriptionally active chromatin.
    Biochem Biophys Res Commun. 1979 Jul 27;89(2):571-9 PMID: 290356
  39. Subnucleosome particles containing high mobility group proteins HMG-E and HMG-G originate from transcriptionally active chromatin.
    Nucleic Acids Res. 1979 Nov 24;7(6):1525-40 PMID: 503859
  40. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.9.1947
Published
1980-05-10
Pages
1947-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324049
Subset
IM
Grants
NIGMS NIH HHS · GM26702-01 · United States
External Links
PubMed source DOI: 10.1093/nar/8.9.1947 PMC Full Text View journal details
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