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PMID: 440975 Published · ppublish English Journal Article

Comparison of non histone proteins selectively associated with nucleosomes with proteins released during limited DNase digestions.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 953-66

Defer N, Crepin M, Terrioux C, Kruh J, Gros F

Abstract

Cultured mammary cells from GR mouse were used to analyse proteins associated with the mononucleosomes and released by a short micrococcal DNase treatment of nuclei. On metrizamide density gradients, mononucleosomes appear to be heterogeneous according to their content of associated non-histone proteins. Proteins associated with the denser fraction (1.22 - 1.24 g/ml) were analysed by two dimensional electrophoresis and compared to the proteins released by DNase I treatment. All the proteins associated with mononucleosomes were also released by DNase I treatment. It could then be assumed that these proteins are associated with the active part of the genome. Additional proteins were released by micrococcal DNase treatment of the nuclei. They could be involved in a higher order organization of chromatin.

MeSH Terms
Animals Cell Line Chromatin/ultrastructure Chromosomal Proteins, Non-Histone DNA, Neoplasm Deoxyribonucleases Electrophoresis, Polyacrylamide Gel Female Mammary Neoplasms, Experimental Mice Molecular Weight Nucleoproteins
Chemicals
Chromatin Chromosomal Proteins, Non-Histone DNA, Neoplasm Nucleoproteins Deoxyribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Defer N
Crepin M
Terrioux C
Kruh J
Gros F
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.3.953
Published
1979-03-00
Pages
953-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327744
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.3.953 PMC Full Text View journal details
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