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PMID: 6424095 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel DNA polymerase induced by Bacillus subtilis phage phi 29.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8333-42

Watabe K, Ito J

Abstract

A novel DNA polymerase induced by Bacillus subtilis bacteriophage phi 29 has been identified. This polymerase can be separated from host DNA polymerase, by fractionation of extracts prepared from phage infected cells, using phosphocellulose chromatography. The isolated polymerase prefers poly(dA)oligo(dT) as template. The DNA polymerase isolated from the cells infected with a gene 2 temperature sensitive mutant (ts2) showed greater heat-lability than that induced by wild type phi 29. The ts2 DNA polymerase was also thermolabile for its activity in the formation of a covalent complex between phi 29 terminal protein and dAMP, the initiation step of phi 29 DNA replication. These findings indicate that gene 2 is the structural gene for a phi 29 DNA polymerase required for the complex formation step of DNA initiation.

MeSH Terms
Bacillus subtilis/enzymology,genetics Bacteriophages/enzymology,genetics DNA-Directed DNA Polymerase/genetics,isolation & purification Genes Genes, Bacterial Genes, Viral Kinetics Mutation Temperature
Chemicals
DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watabe K
Ito J
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8333
Published
1983-12-10
Pages
8333-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326585
Subset
IM
Grants
NIGMS NIH HHS · GM 28013 · United States
External Links
PubMed source DOI: 10.1093/nar/11.23.8333 PMC Full Text View journal details
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