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PMID: 6416226 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Proteins specified by African Swine Fever virus. IV. Glycoproteins and phosphoproteins.

Archives of virology ·Vol. 77 ·No. 2-4 ·1983-00-00 ·Pages 167-80

Tabarés E, Martínez J, Martín E, Escribano JM

Abstract

African Swine Fever virus infected MS cells labeled with radioactive 14C-amino acids, 32Pi or [3H]-glucosamine were examined by high resolution sodium dodecylsulfate polyacrylamide gel electrophoresis and showed 43 infected cell polypeptides. Twenty-one of these proteins were present in the nuclear fraction of infected cells. At least 22 of the infected cell polypeptides induced antibodies during natural infections in swine. The pattern of infected cell polypeptides modified by incorporation of showed prosthetic groups that at least 8 polypeptides were phosphorylated and at least three specific viral glycoproteins (A, B and C) were detected by immunoprecipitation. The most highly glycosylated polypeptide corresponds to the structural viral protein VP51.

MeSH Terms
African Swine Fever Virus/analysis,physiology Animals Antibodies, Viral/biosynthesis Antigens, Viral/immunology Cell Line Cell Nucleus/analysis Glycoproteins/analysis Haplorhini Iridoviridae/analysis Kidney Phosphoproteins/analysis Viral Proteins/analysis,immunology Viral Structural Proteins
Chemicals
Antibodies, Viral Antigens, Viral Glycoproteins Phosphoproteins Viral Proteins Viral Structural Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tabarés E
Martínez J
Martín E
Escribano J M
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32 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01309265
Published
1983-00-00
Pages
167-80
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01309265 View journal details
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