In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2506-10

Zillig W, Fujiki H, Blum W, Janeković D, Schweiger M, Rahmsdorf H, Ponta H, Hirsch-Kauffmann M

Abstract

After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are phosphorylated by a phage-gene-encoded protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). The phosphorylation occurs on threonine residues and appears site-specific. It is probably the molecular basis of the early transcriptional control.

MeSH Terms

Coliphages/enzymology DNA DNA Viruses/enzymology DNA-Directed RNA Polymerases/immunology,metabolism Electrophoresis, Cellulose Acetate Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Phosphoproteins/biosynthesis Precipitin Tests Protein Kinases/metabolism

Chemicals

Phosphoproteins DNA Protein Kinases DNA-Directed RNA Polymerases

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Zillig W

Fujiki H

Blum W

Janeković D

Schweiger M

Rahmsdorf H

Ponta H

Hirsch-Kauffmann M

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.7.2506

Published

1975-07-00

Pages

2506-10

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432797

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.7.2506 PMC Full Text View journal details

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In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.

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