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PMID: 6776940 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties.

Archives of virology ·Vol. 66 ·No. 2 ·1980-00-00 ·Pages 119-32

Tabarés E, Martinez J, Ruiz Gonzalvo F, Sánchez-Botija C

Abstract

Infection of MS cells with African swine fever virus (ASFV) produces inhibition of protein synthesis which is detectable from 4.5 hours after infection. At least 34 viral polypeptides have been indentified with molecular weights ranging between 9500 and 243,000 daltons. Three of these proteins show affinity for the cell nucleus and nine are in both the nuclear and cytoplasmic fractions. Ten early proteins were found, and most of the structural proteins were late proteins. Most of the proteins are synthesized within the first 8 hours after infection. At least nine proteins induced antibodies in the natural infection. Six of these proteins are structural proteins. The antigenic determinants of VP172, VP162, VP146, VP73, VP34, and IP23.5 are in the primary structure of the proteins.

MeSH Terms
African Swine Fever Virus/analysis,immunology,metabolism Animals Antigens, Viral/immunology Cell Line Electrophoresis, Polyacrylamide Gel Haplorhini Iridoviridae/metabolism Kidney Molecular Weight Protein Biosynthesis Time Factors
Chemicals
Antigens, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tabarés E
Martinez J
Ruiz Gonzalvo F
Sánchez-Botija C
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18 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01314980
Published
1980-00-00
Pages
119-32
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01314980 View journal details
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