Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.

Journal of virology ·Vol. 22 ·No. 1 ·1977-04-00 ·Pages 113-7

Norrild B, Vestergaard BF

Abstract

Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.

MeSH Terms

Antigens, Viral/analysis Electrophoresis, Polyacrylamide Gel Glycoproteins/analysis,immunology Immunoelectrophoresis Immunoelectrophoresis, Two-Dimensional Molecular Weight Peptides/analysis Simplexvirus/analysis,immunology Viral Proteins/analysis,immunology

Chemicals

Antigens, Viral Glycoproteins Peptides Viral Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Norrild B

Vestergaard B F

References (16)

16 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.1.113-117.1977

Published

1977-04-00

Pages

113-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515691

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.1.113-117.1977 PMC Full Text View journal details

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Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.

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