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PMID: 6388571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.

The Biochemical journal ·Vol. 223 ·No. 2 ·1984-10-15 ·Pages 507-17

Darlison MG, Guest JR

Abstract

The nucleotide sequence of a 961 base-pair segment of DNA containing the sdhB gene, which encodes the iron-sulphur protein subunit of the E. coli succinate dehydrogenase, has been determined. The sdhB structural gene comprises 711 base pairs (237 codons, excluding the translational initiator and terminator). It is separated by a 15 base-pair intergenic region from the preceding flavoprotein gene (sdhA) and is the distal gene of an operon that also includes genes (sdhC and D) encoding two hydrophobic subunits, sdhCDAB. The distal end of the sdh operon is linked to the 2-oxoglutarate dehydrogenase gene (sucA) by a complex region of dyad symmetry that is homologous with several potential intercistronic regulatory elements or transcriptional attenuators. The sdhB structural gene encodes a polypeptide of Mr26637 that is strikingly homologous with the iron-sulphur protein subunit of fumarate reductase (38% identity, increasing to 58% when conservative changes are included). Both subunits contain 11 cysteine residues, 10 being conserved in three clusters resembling those found in ferredoxins. This work completes the sequence of a 9897 base-pair segment of DNA containing seven tricarboxylic acid cycle genes encoding three enzymes or enzyme complexes, citrate synthase (gltA), succinate dehydrogenase (sdh), and the 2-oxoglutarate dehydrogenase complex (suc), that are organized thus: gltA-sdhCDAB-sucAB.

MeSH Terms
Amino Acids/analysis Base Sequence Codon Escherichia coli/enzymology,genetics Genes, Bacterial Genetic Code Iron-Sulfur Proteins/analysis,genetics Metalloproteins/analysis Multienzyme Complexes/genetics Succinate Dehydrogenase/genetics
Chemicals
Amino Acids Codon Iron-Sulfur Proteins Metalloproteins Multienzyme Complexes fumarate reductase complex Succinate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Darlison M G
Guest J R
References (44)
44 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2230507
Published
1984-10-15
Pages
507-17
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144325
Subset
IM
Databases
GENBANK
X01070
External Links
PubMed source DOI: 10.1042/bj2230507 PMC Full Text View journal details
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