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PMID: 6344013 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The organization of oligonucleosomes in yeast.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3717-36

Szent-Gyorgyi C, Isenberg I

Abstract

We have developed a method of preparing yeast chromatin that facilitates the analysis of nucleoprotein organization. Yeast chromatin, isolated as an insoluble complex, is digested with micrococcal nuclease and fractionated into major insoluble and soluble fractions. No nucleosomal repeat is seen early in digestion for the insoluble fraction. Nucleosomal complexes of the soluble fraction are excised by nuclease in a distinctive non-random pattern; they are markedly depleted in mononucleosomes. When we analyze the soluble material by high resolution native electrophoresis, we find that the nucleoproteins resolve into two bands for each DNA multimer of the nucleosomal repeat. Our results suggest that there are structural similarities between bulk yeast chromatin and chromatin configurations found in transcribing genes of complex eukaryotes.

MeSH Terms
Animals Cell Fractionation Cell Nucleus/ultrastructure Chickens Chromatin/ultrastructure DNA/blood DNA, Fungal/isolation & purification Electrophoresis, Polyacrylamide Gel Erythrocytes/ultrastructure Micrococcal Nuclease/metabolism Nucleosomes/ultrastructure Saccharomyces cerevisiae/ultrastructure
Chemicals
Chromatin DNA, Fungal Nucleosomes DNA Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Szent-Gyorgyi C
Isenberg I
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.11.3717
Published
1983-06-11
Pages
3717-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325998
Subset
IM
Grants
NIGMS NIH HHS · GM 25794 · United States
External Links
PubMed source DOI: 10.1093/nar/11.11.3717 PMC Full Text View journal details
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