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PMID: 6325155 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5' end of the actively transcribed ovalbumin gene.

The EMBO journal ·Vol. 1 ·No. 2 ·1982-00-00 ·Pages 223-30

Bellard M, Dretzen G, Bellard F, Oudet P, Chambon P

Abstract

We examined the chromatin organizations of approximately 3 kb of DNA in the 5'-end flanking region of the ovalbumin gene in chicken erythrocyte and oviduct cell nuclei. With specific DNA probes and an indirect end-labeling technique, we analysed the pattern of the DNA fragments obtained after micrococcal nuclease digestion and generated comparative maps of the nuclease cuts. This region of the chicken genome displays a "typical" chromatin arrangement in erythrocyte nuclei, with nucleosomes apparently positioned at random. In contrast, in oviduct nuclei, the same region has an "altered" chromatin structure, and lacks a typical nucleosomal array. The existence of specifically positioned proteins and of alterations in the DNA secondary structure in this region of the oviduct chromatin is suggested by comparison of the nuclease cleavage maps which reveals specific changes: disappearance of nuclease cuts present in "naked" and erythrocyte chromatin DNAs, and appearance of new cuts absent from these DNAs.

MeSH Terms
Animals Base Sequence Chickens Chromatin/ultrastructure Chromosome Mapping DNA Restriction Enzymes/metabolism Erythrocytes/analysis Female Micrococcal Nuclease/metabolism Ovalbumin/genetics Oviducts/analysis Transcription, Genetic
Chemicals
Chromatin Ovalbumin DNA Restriction Enzymes Micrococcal Nuclease
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bellard M
Dretzen G
Bellard F
Oudet P
Chambon P
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56 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
223-30
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553024
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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