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PMID: 6278445 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6749-62

Wong YC, O'Connell P, Rosbash M, Elgin SC

Abstract

By using an indirect end-labelling technique for mapping, five DNase I hypersensitive sites have been located in Drosophila melanogaster chromatin at the 5'-end of the gene coding for ribosomal protein 49. These sites typically span about 100-600 base pairs and are approximately the length of a nucleosome apart (center to center distance ca 245 bp). This is the first analysis of the chromatin structure of a constitutive house-keeping gene. The results support the hypothesis that the presence of such a DNase 1 hypersensitive site in chromatin is necessary for transcription in vivo. The presence of such sites may reflect some local changes in the conformation of the chromatin in the presumptive regulatory region.

MeSH Terms

Animals Binding Sites Chromatin/isolation & purification,metabolism Cloning, Molecular Deoxyribonuclease I Deoxyribonucleases Drosophila melanogaster/genetics Endonucleases Gene Expression Regulation Molecular Conformation Ribosomal Proteins/genetics

Chemicals

Chromatin Ribosomal Proteins ribosomal protein 49 Deoxyribonucleases Endonucleases Deoxyribonuclease I

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wong Y C

O'Connell P

Rosbash M

Elgin S C

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.24.6749

Published

1981-12-21

Pages

6749-62

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327638

Subset

External Links

PubMed source DOI: 10.1093/nar/9.24.6749 PMC Full Text View journal details

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DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene.

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