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PMID: 739986 Published · ppublish English Journal Article

Separation of nucleosomes containing histones H1 and H5.

Molecular biology reports ·Vol. 4 ·No. 3 ·1978-10-16 ·Pages 185-9

Bakayeva TG, Bakayev VV

Abstract

Hen erythrocyte chromatin was digested with staphylococcal nuclease and fractionated by electrophoresis in polyacrylamide gels. Instead of the three bands described for mouse carcinoma chromatin, four main discrete components (MN1, MN2, MN2E and MN3) were resolved in the mononucleosome fraction of erythrocyte chromatin. MN2 contained all five histones and a DNA fragment of 165--180 base pairs. MN2E comprised four nucleosomal histones plus histone H5 (but not H1) and a DNA fragment of 170--190 base pairs. The relatively nuclease resistant MN3 fraction of erythrocyte nucleosomes contained H1 but no H5 histone. A more accurate analysis of the MN2 fraction in mouse carcinoma nucleosomes revealed some additional microheterogeneity depending on the presence of two different subfractions of H1.

MeSH Terms
Animals Cell Separation/methods Chickens Chromatin/ultrastructure Electrophoresis, Polyacrylamide Gel/methods Erythrocytes/analysis Female Histones/isolation & purification Micrococcal Nuclease/metabolism Structure-Activity Relationship
Chemicals
Chromatin Histones Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bakayeva T G
Bakayev V V
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21 references, click to expand
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00777522
Published
1978-10-16
Pages
185-9
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00777522 View journal details
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