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PMID: 6336728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Host cell metabolic energy is not required for injection of bacteriophage T5 DNA.

Journal of bacteriology ·Vol. 153 ·No. 1 ·1983-01-00 ·Pages 124-33

Filali Maltouf A, Labedan B

Abstract

The addition of various metabolic inhibitors (uncouplers, cyanide, arsenate, ionophores) separately or together (for example, arsenate and an uncoupler) or even harsher methods of energy depletion did not prevent bacteriophage T5 from injecting its first-step-transfer DNA (a DNA segment 3 micron long) into the cytoplasm of host cells. The same indifference to metabolic energy was observed if first-step-transfer DNA was decapsidated and uncoiled before injection, thus precluding any energetic help from the phage capsid or from some tension stored in DNA tightly packed in the head. Penetration of the second-step-transfer DNA across the cytoplasmic membrane was studied by determining injection of superinfecting T5 A2- amber phages into Sup- bacteria containing proteins A1 and A2 previously encoded by the first-step-transfer DNA of a primary wild-type phage. The addition of various metabolic inhibitors after synthesis of proteins A1 and A2 but before superinfection did not prevent this penetration of second-step-transfer DNA. Thus, we conclude that traversal of the cytoplasmic membrane by the entire T5 DNA (a molecule 34 micron long) must occur by diffusion through protein channels.

MeSH Terms
Adenosine Triphosphate/metabolism Arsenates/pharmacology DNA, Viral/metabolism Diffusion Energy Metabolism Escherichia coli/metabolism Hydrogen-Ion Concentration Potassium/metabolism T-Phages/metabolism Uncoupling Agents/pharmacology Viral Proteins/metabolism
Chemicals
Arsenates DNA, Viral Uncoupling Agents Viral Proteins Adenosine Triphosphate arsenic acid Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Filali Maltouf A
Labedan B
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.1.124-133.1983
Published
1983-01-00
Pages
124-33
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217349
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.153.1.124-133.1983 PMC Full Text View journal details
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