Penetration into host cells of naked, partially injected (post-FST) DNA of bacteriophage T5.

Journal of virology ·Vol. 12 ·No. 2 ·1973-08-00 ·Pages 226-9

Labedan B, Legault-Demare J

Abstract

The experiments presented here suggest that the naked post-FST DNA, attached to the bacteria after a centrifugation at 6,000 x g of Escherichia coli-T5 complexes arrested at the FST stage, can be injected into the host cell if protein synthesis is allowed. This results from the observation that the production of infectious centers by this naked DNA is sensitive to DNase or moderate shearing treatments. The process of injection seems to be the same as that for DNA normally rolled in an adsorbed capsid.

MeSH Terms

Centrifugation, Density Gradient Coliphages/analysis,growth & development,metabolism DNA, Viral/isolation & purification,metabolism Deoxyribonucleases Escherichia coli/metabolism Mutation Viral Proteins/biosynthesis

Chemicals

DNA, Viral Viral Proteins Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Labedan B

Legault-Demare J

References (12)

12 references, click to expand

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Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5.
J Virol. 1971 May;7(5):612-8 PMID: 16789131
MOLECULAR ASPECTS OF DNA TRANSFER FROM PHAGE T5 TO HOST CELLS. I. CHARACTERIZATION OF FIRST-STEP-TRANSFER MATERIAL.
J Mol Biol. 1964 Oct;10:10-8 PMID: 14222884
Location of the first step transfer fragment and single-strand interruptions in T5stO bacteriophage DNA.
J Mol Biol. 1973 Apr 5;75(2):213-34 PMID: 4580675
DNA transfer from phage T5 to host cells: dependence on intercurrent protein synthesis.
Proc Natl Acad Sci U S A. 1965 May;53(5):969-73 PMID: 5222566
First-step-transfer deoxyribonucleic acid of bacteriophage T5.
Bacteriol Rev. 1968 Sep;32(3):227-42 PMID: 4879238
Bacteriophage T5 chromosome fractionation: genetic specificity of a DNA fragment.
Science. 1966 Apr 8;152(3719):208-10 PMID: 12325347
Invasion by bacteriophage T5. III. Stages revealed by changes in susceptibility of early complexes to abortive infection.
Virology. 1961 Oct;15:127-35 PMID: 14462207
Patterns of protein synthesis in Escherichia coli infected by amber mutants in the first-step-transfer DNA of T5.
J Mol Biol. 1970 Feb 28;48(1):133-43 PMID: 4915289
Pre-early proteins of bacteriophage T5: structure and function.
J Mol Biol. 1971 Dec 28;62(3):551-64 PMID: 5136582
Functions of two genes in the first-step-transfer DNA of bacteriophage T5.
J Mol Biol. 1969 Aug 28;44(1):173-83 PMID: 4897799
Invasion by bacteriophage T5. II. Dissociation of calcium-independent and calcium-dependent processes.
Virology. 1960 Apr;10:514-29 PMID: 14414046

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.2.226-229.1973

Published

1973-08-00

Pages

226-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356616

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.2.226-229.1973 PMC Full Text View journal details

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Penetration into host cells of naked, partially injected (post-FST) DNA of bacteriophage T5.

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