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PMID: 41245 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Requirement for membrane potential in injection of phage T4 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4669-73

Labedan B, Goldberg EB

Abstract

The first stages of infection by phage T4 may be divided into energy-dependent and energy-independent processes. Irreversible adsorption, unplugging, and initial exposure of the DNA terminus may occur at 4 degrees C, or at 37 degrees C in bacteria whose energy-yielding metabolism has been poisoned. DNA injection into the cytoplasm needs higher temperatures and energy from the host cell. The nature of this energy requirements was deduced from the use of metabolic inhibitors. Our results show that T4 DNA injection specifically requires the presence of a protonmotive force across the cytoplasmic membrane of the host. Moreover, the chemical gradient (delta pH) does not appear to be essential, but the membrane potential (delta psi) is required.

MeSH Terms
Biological Transport, Active Colicins/pharmacology DNA, Viral/metabolism Escherichia coli/physiology Hydrogen-Ion Concentration Ions Membrane Potentials/drug effects T-Phages/physiology Temperature Uncoupling Agents/pharmacology Valinomycin/pharmacology Virus Replication
Chemicals
Colicins DNA, Viral Ions Uncoupling Agents Valinomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Labedan B
Goldberg E B
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4669
Published
1979-09-00
Pages
4669-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411642
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4669 PMC Full Text View journal details
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