Charge transfer mediated by nigericin in black lipid membranes.

Journal of bioenergetics ·Vol. 8 ·No. 1 ·1976-02-00 ·Pages 19-26

Toro M, Gómez-Lojero C, Montal M, Estrada-O S

Abstract

Nigericin, in the concentration range (10(-6) M or higher) at which it uncouples intact mitochondria, was found to increase the conductance of black lipid membranes (BLM) by several orders of magnitude. The dependence of the membrane conductance on pH and K+ concentration suggests a mechanism for the transfer of charge mediated by this ionophore based on a mobile dimer with both nigericin molecules protonated and complexed with one K+. This charged complex accounts for the uncoupling effect observed in intact mitochondria.

MeSH Terms

Anti-Bacterial Agents/pharmacology Electric Conductivity Hydrogen-Ion Concentration Membranes, Artificial Mitochondria/drug effects Models, Biological Nigericin/pharmacology Phosphatidylcholines Potassium/pharmacology

Chemicals

Anti-Bacterial Agents Membranes, Artificial Phosphatidylcholines Nigericin Potassium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Toro M

Gómez-Lojero C

Montal M

Estrada-O S

References (29)

29 references, click to expand

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Article Info

Journal

Journal of bioenergetics

Abbr.

J Bioenerg

ISSN

0449-5705

DOI

10.1007/BF01559387

Published

1976-02-00

Pages

19-26

Language

English

Region

England

NLM ID

1301714

Subset

External Links

PubMed source DOI: 10.1007/BF01559387

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Charge transfer mediated by nigericin in black lipid membranes.

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