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PMID: 5774477 Published · ppublish English Journal Article

The action of certain antibiotics on mitochondrial, erythrocyte and artificial phospholipid membranes. The role of induced proton permeability.

The Biochemical journal ·Vol. 111 ·No. 4 ·1969-02-00 ·Pages 521-35

Henderson PJ, McGivan JD, Chappell JB

Abstract

1. The action of the antibiotics enniatin A, valinomycin, the actin homologues, gramicidin, nigericin and dianemycin on mitochondria, erythrocytes and smectic mesophases of lecithin-dicetyl hydrogen phosphate was studied. 2. These antibiotics induced permeability to alkali-metal cations on all three membrane systems. 3. The ion specificity on each membrane system was the same. 4. Enniatin A, valinomycin and the actins did not induce permeability to protons, whereas nigericin and dianemycin rendered all three membrane systems freely permeable to protons. 5. Several differences were noted between permeability induced by nigericin and that induced by gramicidin. 6. The action of all these antibiotics on mitochondrial respiration could be accounted for by changes in passive ion permeability of the mitochondrial membrane similar to those induced in erythrocytes and phospholipid membranes, if it is assumed that a membrane potential is present in respiring mitochondria.

MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Cell Membrane Permeability/drug effects Cesium/metabolism Erythrocytes/drug effects Humans In Vitro Techniques Membrane Potentials Membranes, Artificial Mitochondria, Liver/drug effects,metabolism Oxygen Consumption Phosphatidylcholines Potassium/metabolism Protons Rats Rubidium/metabolism Sodium/metabolism Tyrothricin/pharmacology
Chemicals
Anti-Bacterial Agents Membranes, Artificial Phosphatidylcholines Protons Tyrothricin Cesium Sodium Rubidium Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Henderson P J
McGivan J D
Chappell J B
References (27)
27 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1110521
Published
1969-02-00
Pages
521-35
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187573
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1110521 PMC Full Text View journal details
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