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PMID: 6327643 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection and characterization of Tn2501, a transposon included within the lactose transposon Tn951.

Journal of bacteriology ·Vol. 158 ·No. 3 ·1984-06-00 ·Pages 866-71

Michiels T, Cornelis G

Abstract

The DNA sequence spanning coordinates 9.9 to 16.4 kilobases of the lactose transposon Tn951 ( Cornelis et al., Mol. Gen. Genet. 160:215-224, 1978) constitutes a transposable element by itself. Unlike Tn951 ( Cornelis et al., Mol. Gen. Genet. 184:241-248, 1981), this element, called Tn2501 , transposes in the absence of any other transposon. Transposition of Tn2501 proceeds through transient cointegration and duplicates 5 base pairs of host DNA. Tn2501 is flanked by nearly perfect inverted repeats (44 of 48), related to the inverted repeats of Tn21 ( Zheng et al., Nucleic Acids Res. 9:6265-6278, 1982). Unlike Tn21 , Tn2501 does not confer mercury resistance.

MeSH Terms
Base Sequence Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Lactose/metabolism Plasmids Species Specificity
Chemicals
DNA Transposable Elements DNA Restriction Enzymes Lactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Michiels T
Cornelis G
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.3.866-871.1984
Published
1984-06-00
Pages
866-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215521
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.158.3.866-871.1984 PMC Full Text View journal details
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