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PMID: 6261087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The tetracycline resistance transposons Tn1721 and Tn1771 have three 38-base-pair repeats and generate five-base-pair direct repeats.

Molecular & general genetics : MGG ·Vol. 181 ·No. 1 ·1981-00-00 ·Pages 87-94

Schöffl F, Arnold W, Pühler A, Altenbuchner J, Schmitt R

Abstract

The 10.7 kilobase (kb) tetracycline resistance transposons Tn1721 and Tn1771, isolated from disparate sources, are completely homologous on the basis of heteroduplex analyses. Both transposable elements are capable of forming multiple duplications of a 5.3 kb portion encompassing the resistance genes (tet region). A model accounting for both, recA-independent translocation and recA-dependent amplification, postulates two direct and one inverted repeat as essential constituents of the transposons. DNA sequence analyses of Tn1721 and Tn1771 have substantiated this model. They demonstrated three identical 38 base pair repeats identically in both transposons dividing them into a "minor transposon" and a tet region. Identical sequences of at least 87 base pairs providing recombination "hot spots" for gene duplication have been found at the ends of the repetitious tet region. Translocation of Tn1721 and Tn1771 generates five base pair direct repeats at the respective sites of insertion. On the basis of the heteroduplex molecules and sequences analyzed the two transposons are identical.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial Drug Resistance, Microbial Escherichia coli/drug effects Models, Genetic Nucleic Acid Hybridization Recombination, Genetic Repetitive Sequences, Nucleic Acid Tetracycline/pharmacology
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes Tetracycline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schöffl F
Arnold W
Pühler A
Altenbuchner J
Schmitt R
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339010
Published
1981-00-00
Pages
87-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X01280, X01281, X01282, X01283, X01284, X01285
External Links
PubMed source DOI: 10.1007/BF00339010
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