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PMID: 6253951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences at the ends of the mercury resistance transposon, Tn501.

Nucleic acids research ·Vol. 8 ·No. 9 ·1980-05-10 ·Pages 1933-45

Brown NL, Choi CL, Grinsted J, Richmond MH, Whitehead PR

Abstract

The nucleotide sequences at the ends of the mercury-resistance transposon, Tn501, have been determined. The terminal sequences are inverted repeated sequences 38 nucleotide pairs in length, which differ in 3 nucleotide pairs. The transposon is flanked by directly repeated sequences of 5 nucleotide pairs, originating from a single pentanucleotide sequence in the recipient replicon. There is no obvious homology between recipient replicons at the site of insertion of the transposon. The structures of the ends of Tn501 are compared with those of other transposons and insertion sequences. The use of Tn501 to locate an EcoRI site within a genetically defined sequence of interest is discussed.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics DNA, Recombinant Drug Resistance Escherichia coli/genetics Genes Mercury Replicon
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Recombinant DNA Restriction Enzymes Mercury
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brown N L
Choi C L
Grinsted J
Richmond M H
Whitehead P R
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.9.1933
Published
1980-05-10
Pages
1933-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324048
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.9.1933 PMC Full Text View journal details
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