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PMID: 6316258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of thrC and of the transcription termination region of the threonine operon in Escherichia coli K12.

Nucleic acids research ·Vol. 11 ·No. 21 ·1983-11-11 ·Pages 7331-45

Parsot C, Cossart P, Saint-Girons I, Cohen GN

Abstract

The entire threonine operon (thrABC) of Escherichia coli K12 was cloned, and the nucleotide sequence of the thrC gene and its 3' flanking region was determined. The translation initiation codon was identified by sequencing the N-terminal part of threonine synthase, the thrC gene product. Analysis of the deduced protein sequence (428 amino acid residues) revealed a region of homology, 35 amino acids long, between the three enzymes encoded by the threonine operon. During examination of the nucleotide sequence of the 1045 base pair fragments following the thrC gene, we detected some potential rho-independent and rho-dependent transcription termination signals.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Operon Plasmids Species Specificity Threonine/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Threonine DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parsot C
Cossart P
Saint-Girons I
Cohen G N
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.21.7331
Published
1983-11-11
Pages
7331-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326486
Subset
IM
Databases
GENBANK
J01706, J01707, J01708, J01709, V00360, X00092
External Links
PubMed source DOI: 10.1093/nar/11.21.7331 PMC Full Text View journal details
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