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PMID: 6259626 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.

Nucleic acids research ·Vol. 9 ·No. 2 ·1981-01-24 ·Pages 339-47

Cossart P, Katinka M, Yaniv M

Abstract

We have sequenced a DNA fragment containing the Escherichia coli thrA-thrB junction, the complete thrB gene and the thrB-thrC junction. The intergenic sequence thrA and thrB is only one base pair. The coding region for homoserine kinase is 927 base pairs long. It is followed by 114 base pair segment in an open reading frame predicting that thrC begins just after non-sense codon of thrB. The presence at the end of thrA and of thrB of sequences that can pair with the 3' end of the 16 S ribosomal RNA suggests that reinitiation of translation occurs at the end of the two genes. The deduced aminoacid sequence for homoserine kinase shows no striking homology with aspartokinase I homoserine dehydrogenase I.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Codon DNA Restriction Enzymes Escherichia coli/genetics Genes Genetic Code Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor) Transduction, Genetic
Chemicals
Codon Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) homoserine kinase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cossart P
Katinka M
Yaniv M
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.2.339
Published
1981-01-24
Pages
339-47
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326696
Subset
IM
Databases
GENBANK
J01706, J01707, J01708, J01709, V00360, X00092
External Links
PubMed source DOI: 10.1093/nar/9.2.339 PMC Full Text View journal details
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