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PMID: 390305 Published · ppublish English Journal Article

Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

Molecular & general genetics : MGG ·Vol. 175 ·No. 1 ·1979-08-00 ·Pages 39-44

Cossart P, Katinka M, Yaniv M, Saint Girons I, Cohen GN

Abstract

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed.

MeSH Terms
Base Sequence Coliphages/genetics DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis DNA, Recombinant/analysis DNA, Viral/genetics Escherichia coli/genetics Genes Genetic Vectors Plasmids Recombination, Genetic Threonine/genetics
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Viral Threonine DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cossart P
Katinka M
Yaniv M
Saint Girons I
Cohen G N
References (35)
35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267853
Published
1979-08-00
Pages
39-44
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
M10644
External Links
PubMed source DOI: 10.1007/BF00267853
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