Home LiteratureArticle Details
PMID: 6300791 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence of the E. coli glyA gene.

Nucleic acids research ·Vol. 11 ·No. 7 ·1983-04-11 ·Pages 2065-75

Plamann MD, Stauffer LT, Urbanowski ML, Stauffer GV

Abstract

The nucleotide sequence of the Escherichia coli glyA gene has been determined. The amino acid sequence predicted from the DNA sequence consists of 417 residues. After the coding region there is a 185 nucleotide sequence preceding the proposed transcription termination region for the glyA gene. This region is preceded by a G-C rich sequence that could form a stable stem-loop structure once transcribed, followed by an A-T rich sequence within which transcription appears to terminate. There is a long region of dyad symmetry and numerous smaller symmetrical regions between the site of translation termination and the proposed transcription termination region. These stem-loop structures show remarkable homology with intercistronic elements of other prokaryotic operons and may play a role in the regulation of glyA gene expression.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Codon Escherichia coli/genetics Genes Genes, Bacterial Nucleic Acid Conformation Protein Biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins Codon
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Plamann M D
Stauffer L T
Urbanowski M L
Stauffer G V
References (32)
32 references, click to expand
  1. Control of one-carbon metabolism in a methionine-B12 auxotroph of Escherichia coli.
    Arch Biochem Biophys. 1966 Nov;117(2):405-12 PMID: 5339713
  2. Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.
    J Mol Biol. 1970 Aug;51(3):501-21 PMID: 4321727
  3. Studies on the regulation of one-carbon metabolism. II. Repression-derepression of serine hydroxymethyltransferase by methionine in Escherichia coli 113-3.
    J Biol Chem. 1972 Jan 25;247(2):348-52 PMID: 4550600
  4. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  5. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  6. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  7. Control of serine transhydroxymethylase synthesis in Escherichia coli K12.
    Can J Microbiol. 1974 Jan;20(1):41-7 PMID: 4595735
  8. Regulation of serine transhydroxymethylase activity in Salmonella typhimurium.
    J Bacteriol. 1974 Dec;120(3):1017-25 PMID: 4373434
  9. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  10. Role of methionine in the regulation of serine hydroxymethyltransferase in Eschericia coli.
    J Bacteriol. 1975 Oct;124(1):269-78 PMID: 1100603
  11. Mitomycin C-induced expression of trpA of Salmonella typhimurium inserted into the plasmid ColE1.
    J Bacteriol. 1977 Jan;129(1):388-94 PMID: 318646
  12. Serine transhydroxymethylase from rabbit liver. Sequence of anonapeptide at the pyridoxal-5'-phosphate-binding site.
    Eur J Biochem. 1976 Nov 15;70(2):397-401 PMID: 1009937
  13. A nucleotide sequence from a ribonuclease III processing site in bacteriophage T7 RNA.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):822-6 PMID: 265576
  14. Multiple pathways for primary processing of ribosomal RNA in Escherichia coli.
    J Biol Chem. 1977 May 10;252(9):3064-73 PMID: 323260
  15. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  16. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  17. The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. coli.
    Cell. 1980 Feb;19(2):393-401 PMID: 6153577
  18. Evidence for a sulfhydryl group at the active site of serine transhydroxymethylase.
    J Biol Chem. 1980 Apr 10;255(7):2986-9 PMID: 7358720
  19. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  20. Computer analysis of nucleic acids and proteins.
    Methods Enzymol. 1980;65(1):595-609 PMID: 6154871
  21. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  22. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
    Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
  23. DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.
    Nucleic Acids Res. 1980 Apr 11;8(7):1551-60 PMID: 7001368
  24. Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21 PMID: 7012834
  25. Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.
    Gene. 1981 Jun-Jul;14(1-2):63-72 PMID: 7021324
  26. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes.
    J Mol Biol. 1981 Feb 15;146(1):1-21 PMID: 6167728
  27. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  28. Transcription termination: sequence and function of the rho-independent tL3 terminator in the major leftward operon of bacteriophage lambda.
    Gene. 1982 Mar;17(3):247-58 PMID: 6213446
  29. A novel intercistronic regulatory element of prokaryotic operons.
    Nature. 1982 Aug 19;298(5876):760-2 PMID: 7110312
  30. Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.
    J Biol Chem. 1982 Oct 10;257(19):11639-43 PMID: 6288695
  31. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  32. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.7.2065
Published
1983-04-11
Pages
2065-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325862
Subset
IM
Grants
NIGMS NIH HHS · GM26878 · United States
Databases
GENBANK
J01620, J01621
External Links
PubMed source DOI: 10.1093/nar/11.7.2065 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2