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PMID: 6315405 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Construction of a correlated physical and genetic map of the Klebsiella pneumoniae hisDGO region using transposon Tn5 mutagenesis.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1831-8

de Bruijn FJ, Stroke IL, Marvel DJ, Ausubel FM

Abstract

Multicopy plasmids containing the hisDG region of Klebsiella pneumoniae were mutagenized with transposon Tn5. The resulting plasmids were examined for their ability to complement hisD and hisG mutations in Escherichia coli. The physical location of Tn5 on each of the hisD::Tn5 and hisG::Tn5 plasmids was determined by restriction endonuclease analysis. By combining the two types of data, a precise correlated physical and genetic map of the K. pneumoniae hisDG region was constructed. Based on this analysis, the minimum sizes of the hisD and the hisG genes were calculated to be 1100 bp and 900 bp, respectively. The hisO(P) region was also identified. The insertional specificity of transposon Tn5 was shown to be very low. One unanticipated result was obtained: Tn5 insertions in the plasmid-borne hisG gene were not polar on hisD.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial/analysis DNA Restriction Enzymes DNA Transposable Elements Genes, Bacterial Genetic Complementation Test Genotype Klebsiella pneumoniae/genetics Mutation Phenotype Plasmids
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Bruijn F J
Stroke I L
Marvel D J
Ausubel F M
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49 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1831-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555367
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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