IS2-43 and IS2-44: new alleles of the insertion sequence IS2 which have promoter activity.

Molecular & general genetics : MGG ·Vol. 175 ·No. 1 ·1979-08-00 ·Pages 53-6

Sommer H, Cullum J, Saedler H

Abstract

The sequence of two new IS2 alleles with promoter activity (IS2-43 and IS2-44) is reported. The alleles are identical and are formed by a 17 bp tandem duplication in an AT-rich region of IS2. This created a new RNA polymerase binding site. A mutation was found that increased the frequency of formation of these 17 bp duplications but not of another class of duplications, the "mini-insertions". This suggested that the mechanisms of formation of the two classes of duplications are different.

MeSH Terms

Alleles Base Sequence DNA Transposable Elements DNA, Bacterial/analysis DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Mutation Nucleic Acid Hybridization

Chemicals

DNA Transposable Elements DNA, Bacterial DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sommer H

Cullum J

Saedler H

References (15)

15 references, click to expand

Development of a system useful for studying the formation of unstable alleles of IS2.
Mol Gen Genet. 1979 May 23;173(1):15-21 PMID: 381839
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
Effect of Rho on transcription of bacterial operons.
Nat New Biol. 1973 Feb 28;241(113):260-4 PMID: 4348358
Nucleotide sequence of the transposable DNA-element IS2.
Nucleic Acids Res. 1979 Mar;6(3):1111-22 PMID: 375194
A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.
Nucleic Acids Res. 1978 Oct;5(10):3759-73 PMID: 364417
Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes.
J Bacteriol. 1975 May;122(2):749-63 PMID: 1092667
Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations.
Mol Gen Genet. 1972;115(3):258-65 PMID: 4555679
Mapping and characterization of promoters in bacteriophages fd, f1 and m13.
J Mol Biol. 1975 Feb 25;92(2):261-77 PMID: 1095754
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, XVI. OLIGONUCLEOTIDES AS TEMPLATES AND THE MECHANISM OF THEIR REPLICATION.
Proc Natl Acad Sci U S A. 1964 Feb;51:315-23 PMID: 14124330
Mutations of Bacteria from Virus Sensitivity to Virus Resistance.
Genetics. 1943 Nov;28(6):491-511 PMID: 17247100
IS-elements in microorganisms.
Curr Top Microbiol Immunol. 1976;75:111-52 PMID: 797531
IS2-61 and IS2-611 arise by illegitimate recombination from IS2-6.
Mol Gen Genet. 1979 Oct 3;176(2):233-8 PMID: 393955
DNA sequence of the mini-insertion IS2--6 and its relation to the sequence of IS2.
Nature. 1978 Oct 19;275(5681):611-7 PMID: 360073
IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.
Mol Gen Genet. 1974;132(4):265-89 PMID: 4610339
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267855

Published

1979-08-00

Pages

53-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267855

Analysis Services

IS2-43 and IS2-44: new alleles of the insertion sequence IS2 which have promoter activity.

Contact

WeChat Official Account