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PMID: 6314328 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete DNA methylation does not prevent polyoma and simian virus 40 virus early gene expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 21 ·1983-11-00 ·Pages 6470-4

Graessmann M, Graessmann A, Wagner H, Werner E, Simon D

Abstract

The effect of DNA methylation on polyoma virus and simian virus 40 gene expression was investigated. For this purpose, the cytosines of all C-G dinucleotides of the viral DNAs were methylated by the use of rat liver methylase and the completeness of methylation was verified by dinucleotide analysis and restriction endonuclease treatment. The biological activity of unmethylated and fully methylated DNAs was tested by microinjecting them into tissue culture cells. The functions analyzed included early and late viral gene expression, viral DNA replication, oncogenic transformation efficiency, and virus maturation. No difference in any of these biological functions was observed between methylated and unmethylated DNA. Early gene expression of methylated DNA is not the result of demethylation because viral DNA reextracted from the injected cells, under nonpermissive conditions, retained the methylation pattern of the input DNA. In contrast, viral DNA extracted from transformed cells or from intact virus particles was partially or completely demethylated.

MeSH Terms
Antigens, Viral, Tumor/genetics DNA (Cytosine-5-)-Methyltransferases/metabolism DNA, Viral/genetics,metabolism Gene Expression Regulation Genes, Viral Methylation Microinjections Plasmids Polyomavirus/genetics Simian virus 40/genetics
Chemicals
Antigens, Viral, Tumor DNA, Viral DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Graessmann M
Graessmann A
Wagner H
Werner E
Simon D
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.21.6470
Published
1983-11-00
Pages
6470-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390135
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.21.6470 PMC Full Text View journal details
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