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PMID: 6312085 Published · ppublish English Journal Article

Molecular cloning of integrated Gardner-Rasheed feline sarcoma virus: genetic structure of its cell-derived sequence differs from that of other tyrosine kinase-coding onc genes.

Journal of virology ·Vol. 47 ·No. 3 ·1983-09-00 ·Pages 611-9

Naharro G, Tronick SR, Rasheed S, Gardner MB, Aaronson SA, Robbins KC

Abstract

Gardner-Rasheed feline sarcoma virus (GR-FeSV) is an acute transforming retrovirus which encodes a gag-onc polyprotein possessing an associated tyrosine kinase activity. The integrated form of this virus, isolated in the Charon 21A strain of bacteriophage lambda, demonstrated an ability to transform NIH/3T3 cells at high efficiency upon transfection. Foci induced by GR-FeSV DNA contained rescuable sarcoma virus and expressed GR-P70, the major GR-FeSV translational product. The localization of long-terminal repeats within the DNA clone made it possible to establish the length of the GR-FeSV provirus as 4.6 kilobase pairs. The analysis of heteroduplexes formed between lambda feline leukemia virus (FeLV) and lambda GR-FeSV DNAs revealed the presence of a 1,700-base-pair FeLV unrelated segment, designated v-fgr, within the GR-FeSV genome. The size of this region was sufficient to encode a protein of approximately 68,000 daltons and was localized immediately downstream of the FeLV gag gene coding sequences present in GR-FeSV. Thus, it is likely that this 1.7-kilobase-pair stretch encodes the onc moiety of GR-P70. Utilizing probes representing v-fgr, we detected homologous sequences in the DNAs of diverse vertebrate species, implying that v-fgr originated from a well-conserved cellular gene. The number of cellular DNA fragments hybridized by v-fgr-derived probes indicated either that proto-fgr is distributed over a very large region of cellular DNA or represents a family of related genes. By molecular hybridization, v-fgr was not directly related to the onc genes of other known retroviruses having associated tyrosine kinase activity.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Base Sequence Cloning, Molecular Genes, Viral Humans Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Oncogenes Protein Kinases/genetics Protein-Tyrosine Kinases Recombination, Genetic Retroviridae/genetics Sarcoma Viruses, Feline/genetics Transcription, Genetic
Chemicals
Nucleic Acid Heteroduplexes Protein Kinases Protein-Tyrosine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Naharro G
Tronick S R
Rasheed S
Gardner M B
Aaronson S A
Robbins K C
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46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.3.611-619.1983
Published
1983-09-00
Pages
611-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255301
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.47.3.611-619.1983 PMC Full Text View journal details
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