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PMID: 6264461 Published · ppublish English Journal Article

Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2077-81

Srinivasan A, Reddy EP, Aaronson SA

Abstract

The integrated proviral genome of Abelson murine leukemia virus (A-MuLV) was cloned in lambda gtWES . lambda B bacteriophage after EcoRI endonuclease digestion and enrichment of proviral sequences by sequential RPC-5 column chromatography and agarose gel electrophoresis. Recombinant DNA clones containing a 7.8-kilobase-pair EcoRI insert were shown to have the entire integrated A-MuLV genome with both 5' and 3' ends flanked by mink cellular DNA sequences. This DNA fragment was shown to induce focus transformation upon transfection of NIH/3T3 mouse cells. Moreover, focus-forming virus could be rescued from transformed nonproducer cells upon superinfection with a type C helper virus. A polyprotein of molecular weight 120,000 (p120) containing murine leukemia virus gag gene determinants was invariably deteced by immunoprecipitation analysis of individual transformants induced by the 7.8-kilobase-pair DNA. Molecularly cloned integrated A-MuLV in its infectious form should be of use in elucidating the mechanisms involved in transformation by this virus.

MeSH Terms
Abelson murine leukemia virus/genetics Animals Bacteriophage lambda/genetics Cell Line Cell Transformation, Viral Cloning, Molecular DNA Restriction Enzymes/metabolism DNA, Viral/genetics Defective Viruses/genetics Gene Expression Regulation Genes, Viral Leukemia Virus, Murine/genetics Mice Virus Replication
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Srinivasan A
Reddy E P
Aaronson S A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2077
Published
1981-04-00
Pages
2077-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319286
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.4.2077 PMC Full Text View journal details
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