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PMID: 6311935 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Altered transcription of genes coding for class I histocompatibility antigens in murine tumor cells.

The Journal of experimental medicine ·Vol. 158 ·No. 4 ·1983-10-01 ·Pages 1294-306

Baldacci P, Pozo F, Gisselbrecht S, Kourilsky P

Abstract

Three murine tumors induced by Moloney murine leukemia virus (M-MLV) which exhibited loss of some or all H-2 class I antigens at the cell surface were analyzed at the DNA and RNA level with molecular probes specific of H-2 heavy chains and beta 2-microglobulin sequences. No observable difference could be detected at the DNA level between the tumors and the parent animals. However, a decrease in H-2 mRNA was observed, especially in phenotypically H-2 negative tumor, BM5R, where H-2 transcripts were at least 30-fold less abundant. These results show that an H-2-negative character may result from a general alteration in the transcription of H-2 genes, which could reflect some kind of regulatory process.

MeSH Terms
Animals Cell Line DNA Restriction Enzymes/metabolism Genetic Code H-2 Antigens/genetics Leukemia, Experimental/genetics,immunology Lymphoma/genetics,immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Moloney murine leukemia virus/immunology RNA, Messenger/analysis Thymoma/genetics,immunology Transcription, Genetic beta 2-Microglobulin/genetics
Chemicals
H-2 Antigens RNA, Messenger beta 2-Microglobulin DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baldacci P
Pozo F
Gisselbrecht S
Kourilsky P
References (36)
36 references, click to expand
  1. The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.
    EMBO J. 1983;2(2):239-43 PMID: 11894933
  2. Comparative studies on the induction of transplantation resistance in BALB-c and C57BL-6 mice in three murine leukemia systems.
    Cancer Res. 1967 Oct;27(10):1743-8 PMID: 6064953
  3. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. H-2-like specificities of foreign haplotypes appearing on a mouse sarcoma after vaccinia virus infection.
    Nature. 1976 Jan 22;259(5540):228-30 PMID: 1250351
  6. Further evidence for depression of H-2 and Ia-like specificities of foreign haplotypes in mouse tumour cell lines.
    Nature. 1976 Jun 24;261(5562):705-7 PMID: 947087
  7. The murine sarcoma virus-induced tumor: exception or general model in tumor immunology?
    Adv Cancer Res. 1977;24:1-66 PMID: 66859
  8. Abelson virus-transformed lymphocytes: null cells that modulate H-2.
    Cell. 1977 Nov;12(3):683-90 PMID: 200369
  9. Immunogenetics of cell surface antigens of mouse leukemia.
    Annu Rev Genet. 1977;11:127-60 PMID: 202188
  10. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.
    Nucleic Acids Res. 1978 Dec;5(12):4631-4 PMID: 745990
  11. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2273-7 PMID: 287067
  12. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
    Nucleic Acids Res. 1979 Dec 20;7(8):2081-103 PMID: 523314
  13. T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens.
    J Immunol. 1980 Jun;124(6):2845-53 PMID: 6154739
  14. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  15. Genetic control of sensitivity to Moloney leukemia virus in mice. IV. Phenotypic heterogeneity of the leukemic mice.
    J Immunol. 1980 Oct;125(4):1415-20 PMID: 7410844
  16. Recovery of biologically active spleen focus-forming virus from molecularly cloned spleen focus-forming virus-pBR322 circular DNA by cotransfection with infectious type C retroviral DNA.
    J Virol. 1980 Sep;35(3):710-21 PMID: 6252333
  17. Differential expression of H-2 gene products in tumour cells in associated with their metastatogenic properties.
    Nature. 1980 Nov 13;288(5787):179-81 PMID: 7432518
  18. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  19. Cancer immunology: the search for specificity--G. H. A. Clowes Memorial lecture.
    Cancer Res. 1981 Feb;41(2):361-75 PMID: 7004632
  20. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.
    J Immunol. 1981 Jan;126(1):317-21 PMID: 6935293
  21. Spatially distinct allodeterminants of the H-2Kk molecule as detected by monoclonal anti-H-2 antibodies.
    J Immunol. 1981 May;126(5):1780-5 PMID: 6163817
  22. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.
    Cell. 1981 Apr;24(1):125-34 PMID: 6786753
  23. Multiple sequences related to classical histocompatibility antigens in the mouse genome.
    Nature. 1981 Jun 25;291(5817):673-5 PMID: 6264319
  24. cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2772-6 PMID: 6265910
  25. A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains.
    Cell. 1981 Sep;25(3):683-92 PMID: 6895187
  26. Control of expression of histocompatibility antigens (H-2) and beta 2-microglobulin in F9 teratocarcinoma stem cells.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5754-8 PMID: 6170985
  27. H-2-like genes in the Tla region of mouse chromosome 17.
    Nature. 1982 Jan 14;295(5845):168-70 PMID: 6276757
  28. Absence of significant H-2 and beta 2-microglobulin mRNA expression by mouse embryonal carcinoma cells.
    Nature. 1982 Mar 18;296(5854):260-2 PMID: 6174872
  29. Use of HLA loss mutants to analyse the structure of the human major histocompatibility complex.
    Nature. 1982 Apr 1;296(5856):454-6 PMID: 7063041
  30. Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.
    Nucleic Acids Res. 1982 Feb 11;10(3):1039-49 PMID: 6278432
  31. Gene dosage and gene expression in the HLA region: evidence from deletion variants.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1235-9 PMID: 6951171
  32. Clusters of genes encoding mouse transplantation antigens.
    Cell. 1982 Mar;28(3):489-98 PMID: 6280871
  33. Structure of wild-type and mutant mouse beta 2-microglobulin genes.
    Cell. 1982 Jun;29(2):661-9 PMID: 6180834
  34. Dominant allele-specific regulation of expression of H-2Kk gene products revealed by somatic cell hybridization.
    Somatic Cell Genet. 1982 Sep;8(5):623-34 PMID: 7135165
  35. Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer.
    Nature. 1982 Nov 18;300(5889):231-7 PMID: 6815535
  36. Multiple mRNA species with distinct 3' termini are transcribed from the beta 2-microglobulin gene.
    Nature. 1983 Mar 31-Apr 6;302(5907):449-52 PMID: 6188058
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.158.4.1294
Published
1983-10-01
Pages
1294-306
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187383
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.158.4.1294 PMC Full Text View journal details
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