Home LiteratureArticle Details
PMID: 6265910 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 2772-6

Kvist S, Bregegere F, Rask L, Cami B, Garoff H, Daniel F, Wiman K, Larhammar D, Abastado JP, Gachelin G, Peterson PA, Dobberstein B, Kourilsky P

Abstract

mRNA coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability to hybridize specifically with mRNA coding for H-2K, D, or L antigens. One of these clones, pH-2d-1, carries a 1200-base-pair insert, comprising the noncoding region, including poly(A) at the 3' end and part of the coding region. A partial sequence of the latter region showed extensive homology with the known amino acid sequences of H-2Kb,Kk, and HLA-B7 antigens. From this comparison, it appears that the coding region extends from amino acid 133 in the second domain, through the third domain, to the cytoplasmic COOH-terminal region. A stretch of 24 hydrophobic or uncharged residues, located 31 amino acids from the COOH-terminal end, could represent the segment that spans the membrane. This is followed on the cytoplasmic side of the membrane by a cluster of basic amino acids and a possible phosphorylation site on a threonine residue.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/immunology Dogs Escherichia coli/genetics H-2 Antigens/genetics Lymphoma/immunology Major Histocompatibility Complex Mice Mice, Inbred DBA Neoplasms, Experimental/immunology Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics Rabbits Reticulocytes/metabolism
Chemicals
DNA, Recombinant H-2 Antigens RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Kvist S
Bregegere F
Rask L
Cami B
Garoff H
Daniel F
Wiman K
Larhammar D
Abastado J P
Gachelin G
Peterson P A
Dobberstein B
Kourilsky P
References (37)
37 references, click to expand
  1. Protein-bound phosphorylthreonine in some tissues and organisms.
    Biochim Biophys Acta. 1970 Oct 20;221(1):107-13 PMID: 4919728
  2. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  3. Glycoproteins.
    Annu Rev Biochem. 1972;41:673-702 PMID: 4563441
  4. Biochemical properties and immunochemical-genetic relationships of mouse H-2 alloantigens.
    Biochim Biophys Acta. 1974 Apr 8;344(1):1-25 PMID: 4598853
  5. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  6. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  7. The minimum substrate of cyclic AMP-stimulated protein kinase, as studied by synthetic peptides representing the phosphorylatable site of pyruvate kinase (type L) of rat liver.
    Biochem Biophys Res Commun. 1976 Jun 7;70(3):696-703 PMID: 180994
  8. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  9. The ovalbumin gene. In vitro enzymatic synthesis and characterization.
    J Biol Chem. 1976 Dec 10;251(23):7355-62 PMID: 63459
  10. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  11. Cloning and amplification of rabbit alpha- and beta-globin gene sequences into Escherichia coli plasmids.
    J Biol Chem. 1977 Apr 10;252(7):2209-17 PMID: 321453
  12. The substrate specificity of adenosine 3':5'-cyclic monophosphate-dependent protein kinase of rabbit skeletal muscle.
    Biochem J. 1977 Feb 15;162(2):411-21 PMID: 192223
  13. Orientation of cell-surface antigens in the lipid bilayer of lymphocyte plasma membrane.
    Nature. 1977 Sep 22;269(5626):307-11 PMID: 904685
  14. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  15. Reactions and crossreactions of a rabbit anti-H2 antigen serum.
    Scand J Immunol. 1978 Apr;7(4):265-76 PMID: 77553
  16. Sequence of the COOH-terminal hydrophilic region of histocompatibility antigens HLA-A2 and HLA-B7.
    J Biol Chem. 1978 Aug 10;253(15):5319-24 PMID: 670198
  17. A method for the recovery of DNA from agarose gels.
    Nucleic Acids Res. 1978 Jul;5(7):2321-32 PMID: 673856
  18. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
    Nucleic Acids Res. 1975 Dec;2(12):2365-78 PMID: 802513
  19. A bacterial clone synthesizing proinsulin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727-31 PMID: 358198
  20. The protein products of the murine 17th chromosome: genetics and structure.
    Adv Immunol. 1978;26:147-93 PMID: 99995
  21. Assembly of the Semliki Forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro.
    J Mol Biol. 1978 Oct 5;124(4):587-600 PMID: 712848
  22. Determination of restriction sites and the nucleotide sequence surrounding the relaxation site of ColE1.
    J Mol Biol. 1978 Oct 5;124(4):601-39 PMID: 712849
  23. Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5329-33 PMID: 82966
  24. Phosphorylation in vivo and in vitro of human histocompatibility antigens (HLA-A and HLA-B) in the carboxy-terminal intracellular domain.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6002-6 PMID: 282620
  25. The major histocompatibility complex of the mouse.
    Science. 1979 Feb 9;203(4380):516-21 PMID: 104386
  26. Further structural analysis of the murine H-2Kb glycoprotein using radiochemical methodology.
    Mol Immunol. 1979 Jan;16(1):3-8 PMID: 447367
  27. Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and beta 2-microglobulin.
    Cell. 1979 Aug;17(4):759-69 PMID: 90552
  28. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  29. Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. 2. Sequence determination and search for homologies.
    Biochemistry. 1979 Dec 11;18(25):5711-20 PMID: 518865
  30. Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein.
    Biochemistry. 1980 Jan 22;19(2):306-15 PMID: 6986168
  31. Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1134-8 PMID: 6928664
  32. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  33. Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.
    Nature. 1980 Nov 20;288(5788):236-41 PMID: 6985476
  34. Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.
    Nucleic Acids Res. 1980 Mar 25;8(6):1231-41 PMID: 6253932
  35. Structure of the heavy chain of the H-2Kk histocompatibility antigen.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4239-43 PMID: 6933473
  36. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  37. Structural studies on H-2Db and H-2Kd molecules indicate that murine major histocompatibility b and d haplotype alloantigens share structural features.
    Mol Immunol. 1980 Oct;17(10):1283-91 PMID: 7464832
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.2772
Published
1981-05-00
Pages
2772-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319439
Subset
IM
Databases
GENBANK
J00393
External Links
PubMed source DOI: 10.1073/pnas.78.5.2772 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2