Home LiteratureArticle Details
PMID: 11894933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.

The EMBO journal ·Vol. 2 ·No. 2 ·1983-00-00 ·Pages 239-43

Rosa F, Berissi H, Weissenbach J, Maroteaux L, Fellous M, Revel M

Abstract

The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.

MeSH Terms
Amino Acid Sequence Base Sequence Burkitt Lymphoma Cloning, Molecular Codon, Initiator DNA, Complementary Escherichia coli Humans Molecular Sequence Data Mutagenesis Protein Sorting Signals RNA, Messenger Sequence Analysis, DNA Sequence Homology, Nucleic Acid Tumor Cells, Cultured beta 2-Microglobulin/genetics
Chemicals
Codon, Initiator DNA, Complementary Protein Sorting Signals RNA, Messenger beta 2-Microglobulin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rosa F
Institut Pasteur, Université Paris VII, France.
Berissi H
Weissenbach J
Maroteaux L
Fellous M
Revel M
References (32)
32 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells.
    Nature. 1982 Oct 28;299(5886):833-6 PMID: 6290893
  3. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  4. The complete amino acid sequence of beta 2-microglobulin.
    Biochemistry. 1973 Nov 20;12(24):4811-22 PMID: 4586824
  5. Production of beta 2-microglobulin by normal and malignant human cell lines and peripheral lymphocytes.
    Transplant Rev. 1974;21(0):53-84 PMID: 4139789
  6. Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon.
    J Gen Virol. 1975 Aug;28(2):207-17 PMID: 170370
  7. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  8. Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines.
    Int J Cancer. 1976 Nov 15;18(5):639-52 PMID: 62724
  9. Molecular genetics of human hemoglobin.
    Annu Rev Genet. 1976;10:157-78 PMID: 797307
  10. Cloning and amplification of rabbit alpha- and beta-globin gene sequences into Escherichia coli plasmids.
    J Biol Chem. 1977 Apr 10;252(7):2209-17 PMID: 321453
  11. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  12. The genetic control of HLA-A and B antigens in somatic cell hybrids: requirement for beta2 microglobulin.
    Tissue Antigens. 1978 Feb;11(2):96-112 PMID: 77067
  13. Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5329-33 PMID: 82966
  14. How do eucaryotic ribosomes select initiation regions in messenger RNA?
    Cell. 1978 Dec;15(4):1109-23 PMID: 215319
  15. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2273-7 PMID: 287067
  16. Molecular cloning and nucleotide sequence of the human growth hormone structural gene.
    Nucleic Acids Res. 1979 Sep 25;7(2):305-20 PMID: 386281
  17. Cell-free synthesis and segregation of beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3651-5 PMID: 91168
  18. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA.
    Cell. 1980 Mar;19(3):671-81 PMID: 6985473
  19. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  20. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  21. Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase.
    Nature. 1980 Nov 6;288(5786):98-100 PMID: 6159548
  22. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  23. The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation.
    Cell. 1981 Feb;23(2):561-71 PMID: 7471213
  24. Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):554-8 PMID: 6165997
  25. Interferon enhances the amount of membrane-bound beta2-microglobulin and its release from human Burkitt cells.
    Eur J Immunol. 1981 Jun;11(6):524-6 PMID: 6167453
  26. K562 cells express human major histocompatibility antigens.
    Immunogenetics. 1981;13(4):359-65 PMID: 6168583
  27. Human leukocyte interferon: isolation and characterization of several molecular forms.
    Arch Biochem Biophys. 1981 Aug;210(1):307-18 PMID: 6170262
  28. Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613-7 PMID: 6171820
  29. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3082-6 PMID: 6179076
  30. Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5' end of mRNA.
    Nature. 1982 Sep 16;299(5880):221-6 PMID: 6955600
  31. Structure of wild-type and mutant mouse beta 2-microglobulin genes.
    Cell. 1982 Jun;29(2):661-9 PMID: 6180834
  32. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.
    Cancer Res. 1968 Jul;28(7):1300-10 PMID: 4174339
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
239-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555120
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2